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Description
FLAG Tag Recombinant Rabbit mAb (FITC Conjugate) (S-589-8)Product Specification Host Rabbit Antigen FLAG Tag Immunogen Synthetic Peptide Clone Number S 589 8 Antibody Type Recombinant mAb Isotype IgG Application ICC, ICFCM Reactivity Species Independent Positive Sample H3 Flag V5 transfected 293T cells Purification Protein A Concentration 2 mg ml Conjugation FITC Physical Appearance Liquid Storage Buffer PBS, 25% Glycerol, 1% BSA, 0. 3% Proclin 300 Stability & Storage 12 months from date of receipt
Product Specification
| Host | Rabbit |
| Antigen | FLAG Tag |
| Immunogen | Synthetic Peptide |
| Clone Number | S-589-8 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, ICFCM |
| Reactivity | Species Independent |
| Positive Sample | H3-Flag-V5 transfected 293T cells |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:500 | Species Independent |
| ICFCM | 1:800 | Species Independent |
Background
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a peptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). It is one of the most specific tags and it is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells. FLAG-tag has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. FLAG-tag can also be used in the isolation of protein complexes with multiple subunits.
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