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Description
Human Bruton'S Tyrosine Kinase ELISA KitProduct Specification Usage Sample collection, processing and storage methods: 1, serum: use do not contain pyrogen and endotoxin in vitro, in the process of operation to avoid any cell stimulation, collect blood after 3000 turn centrifugal 10 minutes to separate serum and erythrocyte quickly carefully. 2, plasma: EDTA, citric acid salt or heparin anticoagulation. The supernatant was removed by centrifugation at 3000 RPM for 30 min. 3, on the cell
Product Specification
| Usage | Sample collection, processing and storage methods: 1, serum: use do not contain pyrogen and endotoxin in vitro, in the process of operation to avoid any cell stimulation, collect blood after 3000 turn centrifugal 10 minutes to separate serum and erythrocyte quickly carefully. 2, plasma: EDTA, citric acid salt or heparin anticoagulation. The supernatant was removed by centrifugation at 3000 RPM for 30 min. 3, on the cell supernatant: 3000 turn 10 minutes of centrifugal to get rid of particle and polymer. 4. Tissue homogenate: add appropriate amount of normal saline to the tissue and mash it. 3000 turn centrifugal take supernatant 10 minutes. 5. Storage: if the sample is not detected in time after collection, please pack according to the dosage, freeze at -20℃, avoid repeated freezing and thawing, thaw at room temperature and ensure that the sample is evenly and fully thawed. Bring your own items: 1. Microplate reader (450nm) 2 washer, high precision and spear: 0.5-10 ul, 2-20 ul, 20-200 - ul, ul 200-1000 3, 37℃ incubator The reagent preparation: Dilution of 20× wash buffer: Distilled water was diluted 1:20, that is, 1 part of 20× wash buffer was added to 19 parts of distilled water. Washing plate method: 1, hand washing board: jilt liquid in the hole, every hole filled with liquid detergent. Let stand for 1 min after the left hole all liquid, pat dry on blotting paper, so wash board 5 times. 2. Automatic washing machine: inject 350μL of washing solution into each well, soak for 1min, and wash the plate 5 times. Operation steps: 1. Remove the required slats from the aluminum foil bag after 20min of equilibrium at room temperature, and the remaining slats are sealed with a ziplack and placed back at 4 ° C. Bore 2, set up the standard and sample, standard holes with different concentrations of standard 50 mu L; 3. Add 10μL sample to the sample well, and then add 40μL sample diluent; Blank holes are not added. 4, in addition to the blank hole, bore standard and sample of each hole to join horseradish peroxidase (HRP) labeled 100 mu L detection antibody, seal plate film sealed reaction with hole, 37 ℃ water bath pot or constant temperature box temperature 60 min. 5, abandon to liquid, pat dry on the blotting paper, every hole filled with liquid detergent. Let stand for 1 min, jilt to detergents, pat dry on the blotting paper, repeat 5 times wash plate (also available microplate wash plate). 6, 50μL of substrate A and B were added to each well and incubated at 37 ° C in the dark for 15min. 7, 50 mu, L per hole to join terminated liquid within 15 min, determine the hole in the 450 - nm wavelength, OD value. Results: Drawing standard curve: in Excel worksheets, with standard concentration as the abscissa, ordinate corresponding OD value, draw the standard linear regression curve, the curve equation of the sample density is calculated. |
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| Theory | Double-antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA) was used. Samples, standards, and HRP-labeled detection antibodies were added to the coated microwells precoated with Bruton's tyrosine kinase (Btk) antibody in turn, then incubated and washed thoroughly. After incubation and thorough washing, the substrate TMB was used for color development. TMB was converted to blue under the catalysis of peroxidase and to yellow under the action of acid. The degree of color is positively correlated with the level of Bruton's tyrosine kinase (Btk) in the sample. The activity of the samples was calculated by measuring the absorbance (OD) value at 450nm using a microplate reader. | |||||||||||||||||||||||||||||||||
| Detection Type | Serum, cell supernatant, and tissue homogenate urine | |||||||||||||||||||||||||||||||||
| Description |
Bruton's tyrosine kinase (Btk or BTK for short), also known as tyrosine protein kinase BTK, is a tyrosine kinase encoded by the BTK gene. BTK plays a crucial role in B cell development. BTK contains five different protein interaction domains. These domains include an amino-terminal pleckstrin homology (PH) domain, a proline-rich TEC homology (TH) domain, the SRC homology (SH) domains SH2 and SH3, and a kinase structure with enzymatic activity. BTK plays a critical role in B cell development because it is required to transmit signals from pre-B cell receptors formed after successful immunoglobulin heavy chain rearrangement. Btk contains a PH domain that binds to phosphatidylinositol-triphosphate (PIP3). Binding of PIP3 induces Btk to phosphorylate phospholipase C, which in turn hydrolyses PIP2 (a phosphatidylinositol) to two second messengers, inositol triphosphate (IP3) and diacylglycerol (DAG), which then regulate the activity of downstream proteins during B-cell signaling. kit performance: |
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| Composition |
Note: The concentrations of standard substance (S0-S5) were 0, 75, 150, 300, 600, 1200 U/mL |
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| General Notes | 1. The kit was stored at 2-8 ° C and equilibrated at room temperature for 20 minutes before use. The concentrated washing solution removed from the refrigerator will have crystallization, which is a normal phenomenon, and the water bath will be heated to completely dissolve the crystals before use. 2. The slats that are not used in the experiment should be put back into the ziplock bag immediately, sealed (dry at low temperature) and stored. 3, concentration of 0 S0 standard can be regarded as negative control or blank; When operating according to the instructions, the sample has been diluted 5 times, and the final result multiplied by 5 is the actual concentration of the sample. 4, in strict accordance with the instructions indicated in the time, the amount of liquid and the order of the incubation operation. 5, all liquid components shake well before use. |
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| Storage Temp. | 2-8℃, protected from light and moisture, valid for 6 months. |
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| Test Range | 0.625-20 ng/mL |
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4.0 ★★★★★
Based on 7 reviews
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Product Reviews
★★★★★ 3
Notes on Legal Style by a Law Professor and Experienced Lawyer.
Format: Kindle
BOOK REVIEW: MEYER, Philip N., Storytelling for Lawyers
ISBN: 978-0-19-5396638
Read June, 13th-27th, 2017.
This book discusses storytelling tools by presenting a series of examples of good storytelling, both in legal settings and in literary works and movies. If theoretical explanations are sometimes a bit dry, the frequent quoting of practical examples conveys fluidity and speed to the book.
After an introduction presenting lawyers as storytellers, it deals with the roles played in storytelling by Plots (chapters 2 and 3); Character (4 and 5); Voice, Perspective, Details and Images, and Rhytm and Speed (which relate to Scene and Summary) (chapter 6); Place or Story Environment (chapter 7) and Narrative Time.
Focusing maybe too narrowly on legal storytelling before American juries, plot is almost equated with melodrama. Films like Jaws and High Noon are extensively discussed, as Gerry Spence’s Closing Argument on Behalf of Karen Silkwood. The chapters on character offer interesting insights on character classification (“round” characters, with psychological depth, prone to suffer transformation as the story evolves, vs. “flat” ones), while discussing the tools for telling how a character is, as opposed to simply showing the psychological nature of each character’s character through dialogue or the actions the character performs. Examples include Tobias Wolff’s This Boy’s Life and Jeremiah Donovan’s Closing Arguments on Behalf of Louis Failla, in a 13-week trial the Author could scrupulously attend in person.
Discussions on Voice, Perspective, Details and Images, Scene and Summary, criticize the basic assumptions of the neutrality of lawyers’ voices, exemplifies how to manage details to suggest ideas and emotions, draw on the distinction between showing and telling, and offers interesting insights into the narrative theory’s concept of stretch (the slowing of the narrative rhythm in relation to the narrated story’s). Environment depiction storytelling tools deals with Joan Didion’s The White Album and the Judicial Opinion in a Rape Case, quoting also from W. G. Sebald’s The Emigrants and the Petition Briefs in Reck v. Ragen and Miranda v. Arizona. Further examples are Kathryn Harrison’s While They Slept and the Petitioner’s Brief in Eddings v. Oklahoma.
Finally, the chapter on Narrative Time draws on Kurt Vonnegut’s Slaughterhouse Five and explores time, rhythm or speed, discussing more deeply stretch and the relation of time of the narrative itself with the time of the facts dealt with in the narrative. Chronology is discussed and criticized; Analepsis or Flashback is didactically explained and exemplified, both in general storytelling theory and in its legal use; the same holds for Prolepsis (Flash-forward) and Ellipsis (the intentional omission of a part of the narrative, often with the purpose of emphasizing the omitted event. Pacing and Rhythm are discussed in more lenght, with the caveat - repeated somewhat throughout the book - that legal stories are often left unfinished by the lawyer, in order to allow the jurors or judges fill the end with their decision.
The Author remarks his purpose was to suggest possible tools and ways of dealing with problems which arise in legal storytelling, and he delivers what he promises.
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Reviewed in the United States on June 27, 2017
★★★★★ 5
Great book and great professor
Format: Paperback
Professor Meyer is a great writer. I had took his death penalty case at Vermont Law School. He writes for numerous magazines including the ABA. I would highly recommend this book and all of his writings.
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Reviewed in the United States on January 19, 2021
★★★★★ 4
Interesting book
Format: Paperback
I am not a lawyer, nor a writer, but rather a reader. I found the correlation of legal storytelling with sceenplay, literary narrative quite interesting. Legal trials are theater.
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Reviewed in the United States on March 20, 2014
★★★★★ 5
Highly recommended -- not just for lawyers!
Format: Paperback
I'm not a lawyer but a Classics professor looking for modern parallels to (and contrasts with) Cicero's persuasive strategies in Roman courts. This book was just what I was looking for: lucid, informative, smart, and as a bonus, well versed in narrative theory, which Meyer handles as an experienced teacher -- avoiding jargon and needless complication, illustrating the key ideas with well-known cinematic examples.
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Reviewed in the United States on April 20, 2017
★★★★★ 5
Super overview and commentary on the historiy and many iterations/amendments
Format: Hardcover
I purchased and carefully read Jeff Kosseff's "The Twenty-Six Words That Created the Internet." It is an illuminating and thorough account of all facets of the legislation including history, enactment, modifications and amendments etc. A complex subject to understand, Mr. Kosseff did an outstanding job of presenting the complicated history and details and understanding of why this is incredibly important leading up to the form that is now in use. Some reviewers complain about too much detail, but I disagree, it is very important to understand the detail. A difficult topic and a great job of presentation. I would like to have a time line e.g., something like a "herring bone" chart notating all of the critical stages of development up to the present ... from initial concepts to Section 230 rollout and the important changes / amendments in both substance and use of 230. I am now reading Koseff's latest book on a similar but different topic, "The United States of Anonymous" which deals with how the first amendment shaped the development of our online speech.
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Reviewed in the United States on March 16, 2023