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Description
Mouse SLC30A6 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Solute Carrier Family 30, Member 6 (SLC30A6) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Solute Carrier Family 30, Member 6 (SLC30A6) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Solute Carrier Family 30, Member 6 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Zinc transporter 6, also known as ZNT6/SLC30A6, is an enzyme encoded by the ZNT6/SLC30A6 gene. This gene encodes a member of the zinc transporter protein family. This protein regulates subcellular zinc levels in the Golgi apparatus and vesicles. Its expression is altered in Alzheimer's disease brain plaques. Diseases associated with SLC30A6 include autosomal dominant spastic paraplegia 4 and zinc-deficiency acrodermatitis enteropathicum. Pathways involved include metal ion SLC transporters and peptide hormone metabolism. An important paralog of this gene is SLC30A5. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.2 ★★★★★
Based on 23 reviews
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Product Reviews
★★★★★ 5
Great Serta Chair
Color: Black
Great chair. Definitely need two people to assemble. Heavy!!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 2, 2026
★★★★★ 5
Comfort and Support
Color: Grey
If you're looking for an office chair with comfort and support in mind, then this is one you should consider. Item delivered on time as stated when purchased. Assembly instructions were quite clear, just make sure all parts are accounted for when opening box. Pay attention to the supplied screw sizing during assembly per the instructions. The supplied hex key, if used, will get the assembly job done, but would recommend using a cordless drill with clutch and appropriate sized hex bit for faster and less fatiguing hand work. Just be sure to start screws by hand first to make sure they start threading into the openings so you won't cross thread the screws. Once assembled, set the chair adjustments to your liking and enjoy a very nice looking, comfortable, and supportive office chair. I especially like the lumbar support built into this product. One last note, if you are going to use this product on hard surfaced floors it is recommended that you purchase soft office chair wheels (sold here on Amazon) because the wheels supplied are hard plastic and will scratch or mar hard flooring.
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Reviewed in the United States on March 6, 2020
★★★★★ 5
Exceptional value!!!
Color: White
The chair is of high quality and is easy to put together, even for a mechanically challenged person like me!! I purchased and assembled two of them!! It is very attractive and we have gotten compliments from those who have seen them. It is extremely comfortable and I would highly recommend.
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Reviewed in the United States on June 7, 2026
★★★★★ 5
Great chair!
Color: Black
Had some issues with Delivery! But got the chair. Beautiful and comfortable. Had one once before as a gaming chair, same kind but bought it from walmart. I used to fall asleep in it all the time. Now i have it for work! One of the best chairs for the cheapest price you can get! If you want a more comfortable chair then this..... your looking at about 300 bucks. Which is next on my list!
It comes with its own screws and washers. The manual is self explanatory however you do kinda gotta play the guessing game. It's pretty simple! match the screws up with the ones in diagram. It comes with an Alan wrench. Your hand might be a bit tired tho. I advise to tighten them with super lock tight or gorilla glue so they never come unloose. And gorilla glue the bolt covers on.
Keep in mind if you use gorilla glue..... they will never come off. Lol. But as far as tighting it.... do not over tighten it. Tighten enough to where you have to put some pressure on it to turn it half way and stop.
Another tip, it's a bit of a pain to set it up with one person. If you got an extra set of hands... use em. If not.... you might be their for a bit.
Start with setting up base.
Then put arms on seat.
(Keep bolts loose but screwed in. You'll see why later when you tighten them down)
Then attach back to arms and seat.
(Now make sure bolts are in holes and tighten them down. But not at once! Do about 4 turns, move to another one. Then the other side. It's like tightening lug nuts on the wheel of a car. If you tighten one all the way down it will throw all the other bolts off and make it much more difficult to ease the bolts into the holes they go in)
Then attach seat to base.
Believe me. It's way easier if you do it like that instead of how the installation instructions say.
After that, put the caps on the holes to cover the bolts. If you don't glue them, they will pop off ..... their not..... super good. But!!!! 1 or 2 drops of gorilla glue and you never have to worry about them popping off. (They seem like they go on their tight, but they don't stay on!)
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Reviewed in the United States on March 31, 2024
★★★★★ 4
Nice chair but definitely not breathable.
Color: Black
Once I figured out the arms had a left and right...the chair was easy to put together. The faux leather is nice. The seat is (or will be once I break it in) very comfortable. I have 2 minor complaints. It is extremely straight up and down. I haven't had much chance to play with it to see if I can get it to recline even an inch. That would help. And number two, it's HOT. I'm going to have to get some sort of seat pad because the pleather is not breathable and every time I get up my bum is sweaty. I know, TMI. But all in all I think it will serve my purposes.
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Reviewed in the United States on April 16, 2026