SKU: 6548079762

TNF-α Protein, Human

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Description

TNF-α Protein, HumanProduct Specification Species Human Synonyms TNF; Tumor Necrosis Factor, Cachectin, TNF Alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF a, TNF, TNFA, TNFSF2 Accession P01375 Amino Acid Sequence Val77 Leu233 Expression System CHO Molecular Weight 17 kDa(Reducing) Purity 95%, by SDS PAGE under reducing conditions Endotoxin <0. 1EU g Conjugation Unconjugated Tag No Tag Physical Appearance Lyophilized Powder Storage Buffer PBS, pH7. 4

Product Specification


Species Human
Synonyms TNFα;Tumor Necrosis Factor, Cachectin, TNF-Alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, TNF, TNFA, TNFSF2
Accession P01375
Amino Acid Sequence

Val77-Leu233
Expression System CHO
Molecular Weight

17 kDa(Reducing)
Purity ＞95%, by SDS-PAGE under reducing conditions
Endotoxin <0.1EU/μg
Conjugation Unconjugated
Tag No Tag
Physical Appearance Lyophilized Powder
Storage Buffer

PBS, pH7.4
Reconstitution

Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation .
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

· 3 months, -20 to -80℃ under sterile conditions after reconstitution.

· 1 week, 2 to 8℃ under sterile conditions after reconstitution.

· Please avoid repeated freeze-thaw cycles.

Background

Tumor necrosis factor alpha (TNFa) is a multifunctional proinflammatory cytokine synthesized mainly by nucleated blood 
cells as a 233 aa type II transmembrane protein which is cleaved by ADAM17 between aa 76-77 to form a soluble 
homotrimeric complex. TNFa plays a role in lipid metabolism, coagulation, and endothelial function and has been 
associated with cancer, infection and inflammation (including inflammatory bowel disease), ischemia/reperfusion injury 
and heart failure, and insulin resistance.

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SKU: 6548079762

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4.3 ★★★★★
Based on 22 reviews
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Product Reviews
M
Verified Purchase
MaryBeth K
Battle Creek, US
★★★★★ 5
Fae Courts with High Intrigue
Format: Kindle
This book is one that just builds and builds and then surprises you to no end. You may think you know the villains and then you are jolted in another direction. Princess Reyna is a real gem, strong of character, a fierce fighter, and loyal to her family and kingdom. Just when you think she and Lorcan, well you know, the plot is flipped. Can't wait to see where this goes in book two.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 7, 2023
K
Verified Purchase
Kindle Customer Maureen
Lake Worth, US
★★★★★ 4
Slow, sorry but good
Format: Kindle
This was a slow moving book. Lots of character pov chapters, lots of superfluous descriptions and endless courtly appearances stalled this book to start. Once you get into the heart of the story, it takes off. Before you know it the book is done. My favorite character is Reyna. She is so strong. She is true to herself. She gets into a lot of trouble with her headstrong ways but it's entertaining. I have high hopes for Lorcan. He is honorable to a fault. Thane had turned out to be better than I thought but i still don't like him. Eislin is useless. Great plot twists at the end. I'm looking forward too book 2.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 6, 2020
F
Verified Purchase
Faifre6
Alexandria, US
★★★★★ 5
Very detailed! Beautiful world building! Strong Heroine!
Format: Kindle
Starts off a little slow and confusing with different POV’s, but starts to all come together towards the middle to make an elaborate plot line and makes it all worth it. Beautiful world building and attention to detail as well as great writing. The cliffhanger was gut wrenching! Can’t wait for the next book!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 4, 2020
I
Verified Purchase
Isabelle
Louisville, US
★★★★★ 4
Interesting
Format: Kindle
This was a very captivating book once you got into it thoroughly. But the third person perspective was a bit hard to get used to. But as you got into it and followed the different characters, it was interesting and filled with intrigue, conflict and forbidden love. I can’t wait to read the next one and to complete the series.
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Reviewed in the United States on November 12, 2022
B
Verified Purchase
Busy Book Bee 📚
Charlottesville, US
★★★★★ 5
I LOVE this book so much!!!!!
Format: Kindle
WOW what a ride!!! To Bleed A Kingdom is Ella Dawes debut book and I am thoroughly impressed by her writing! She writes like a seasoned author already! I absolutely LOVE her writing style it’s fun easy to follow and very imaginative and expressive where a reader can actually FEEL what the characters are going through or have gone through. This world and the characters in it are so very relatable so real feeling like they could actually be my family and friends and enemies also lol The mystery surrounding Lena her friends or found family and their lives is not reveled wholly in this book we get pieces and I really loved that, normally I’m very impatient but with the way this world and all the characters are written it doesn’t become so much the main focus. We get a really solid feel for this world and all it’s going on and people in it. New friendships and love interests are formed it’s a very character driven story which I personally love!! Lena is beautifully broken and flawed but sooooo incredibly strong without the unnecessary stubbornness we come across in so many books and what a breath of fresh air that was to read! Darius is an Alpha male through and through with a LOT of emotional trauma but he loves and he loves hard even when he doesn’t really realize it himself. The side characters are phenomenal the dialogue is sooo good the banter is hilarious and the loyalty and love between them all is written beautifully! The spice is little but it’s amazing when it happens wink wink I don’t like cliffhangers especially when I’m not sure when the next book will be released I’ve been burned too many times but once I got started reading this I didn’t stop all day and late into the night so I’m going to have find my patience and wait and I am so very excited to see where Ella takes this story. Thank you for sharing this story with us can’t wait for more!!! I read this on KU
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Reviewed in the United States on March 29, 2024

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