SKU: 68066307535

Human FlT-3L ELISA Kit

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Description

Human FlT-3L ELISA KitProduct Specification Usage Sample handling and requirements 1. Serum: The whole blood sample collected in the serum separation tube is placed at room temperature for 2 hours or 4 overnight, and then centrifuged at 1000 g for 20 minutes. The supernatant can be taken, or the supernatant can be stored at 20 or 80 , but repeated freezing and thawing should be avoided. 2. Plasma: Specimens were collected using either EDTA or heparin as anticoagulant and

Product Specification

Usage

Sample handling and requirements

1.
Serum:The whole blood sample collected in the serum separation tube is placed at room temperature for 2 hours or 4 ℃ overnight, and then centrifuged at 1000 × g for 20 minutes.
The supernatant can be taken, or the supernatant can be stored at-20 ℃ or-80 ℃, but repeated freezing and thawing should be avoided.

2.
Plasma:Specimens were collected using either EDTA or heparin as anticoagulant and subjected to the specimens within 30 minutes after collection at 2-8 °C 1000
× g centrifuge for 15 minutes, take the supernatant for detection, or store the supernatant at-20 ℃ or-80 ℃, but avoid repeated freezing and thawing.

3.
Tissue homogenate:The tissues were washed with pre-cooled PBS (0.01 M, pH = 7.4) to remove residual blood (lysed red blood cells in the homogenate would affect the measurement results), and the tissues were sheared after weighing.
Combine the chopped tissue with the corresponding volume of PBS (generally according to the weight-to-volume ratio of 1: 9, for example, 1g of tissue sample corresponds to 9mL of PBS.
The specific volume can be appropriately adjusted according to the needs of the experiment and recorded.
It is recommended to add protease inhibitor to PBS) add to a glass homogenizer and grind thoroughly on ice.
For further lysis of tissue cells, the homogenate can be sonicated, or freeze-thawed repeatedly.
Finally, the homogenate was centrifuged at 5000 × g for 5-10 minutes, and the supernatant was taken for detection.

4.
Cell culture supernatant or other biological specimen:Please centrifuge at 1000 × g for 20 minutes, take the supernatant for detection, or store the supernatant at-20 ℃ or-80 ℃, but avoid repeated freezing and thawing.

Note: Hemolysis of the specimen will affect the final test result, so hemolyzed specimens should not be tested.

Reagent Preparation

The kit should be removed from the refrigerated environment and equilibrated at room temperature before use.

Dilution of 20 × wash buffer: Distilled water was diluted 1:20, i.e. 1 part of 20 × wash buffer plus 19 parts of distilled water.

Operation steps

1.
Take out the required slats from the aluminum foil bag after equilibration at room temperature for 20 minutes, and seal the remaining slats with a ziplock bag and put them back to 4 °C.

2.
Set up standard wells and sample wells, and add 50μL of different concentrations of standards to each standard well;

3.
Add 50μL of the sample to be tested to the sample well; Blank holes are not added.

4.
Add horseradish peroxidase (HRP)-labeled detection antibody to each well of the standard well and sample well except for the blank well
100 μL, seal the reaction wells with a plate sealing membrane, and incubate for 60 minutes in a 37 °C water bath or incubator.

5.
Discard the liquid, pat dry on absorbent paper, fill each well with washing liquid (350μL), let it stand for 1min, throw away the washing liquid, pat dry on absorbent paper, and repeat washing the plate 5 times (you can also use a plate washing machine to wash the plate).

6.
Add 50 μL of each substrate A and B to each well, and incubate at 37 °C in the dark for 15 minutes.

7.
Add 50 μL of stop solution to each well, and measure the OD value of each well at a wavelength of 450nm within 15 minutes.

Calculation of experimental results

Taking the OD value of the measured standard product as the abscissa and the concentration value of the standard product as the ordinate, draw the standard curve on coordinate paper or with relevant software, and get the linear regression equation.
Substitute the OD value of the sample into the equation to calculate the concentration of the sample.

Standard curve

Note: Repeatability: The intra-plate coefficient of variation is less than 10% and the inter-plate coefficient of variation is less than 15%.

Species Reactivity Human
Theory The kit uses a double-antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Specimens, standards, and HRP-labeled detection antibodies were sequentially added to coated microwells previously coated with human FMS-like tyrosine kinase 3 ligand (Flt-3L; FLT3LG) capture antibodies, incubated and washed thoroughly. The color is developed with the substrate TMB, which is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. There was a positive correlation between the depth of color and the human FMS-like tyrosine kinase 3 ligand (Flt-3L; FLT3LG) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450nm, and the sample concentration was calculated.
Synonym FLT3LG; FLT3-LG; Flt3-L; FL; Fms-Related Tyrosine Kinase 3 ligand; SL cytokine
Composition

Name

96T remark
Microwell microplate 12 holes × 8 strips without
Standard 0.3 mL * 6 tubes without
Sample dilution 6mL without
Detection Antibody-HRP 10mL without
20 × Wash Buffer 25mL Dilute as per instructions
Substrate A 6mL without
Substrate B 6mL without
Stop liquid 6mL without
Sealing film 2 sheets

without



remark: 1. The standard concentration is 2000, 1000, 500, 250, 125, 62.5 pg/mL in order
  1. After testing a large number of normal specimens, the normal concentration values of the specimens are all within the detection range provided by the kit. During the experiment, 50μL samples can be directly taken and loaded. When some sample values exceed the maximum standard concentration, the sample can be appropriately diluted with sample diluent before conducting the experiment.
General Notes

1. Incubate in strict accordance with the specified time and temperature to ensure accurate results. All reagents must reach room temperature 20-25 °C prior to use. Store reagents in refrigeration immediately after use.

2. Incorrect plate washing can lead to inaccurate results. Make sure to drain the liquid from the wells as much as possible before adding the substrate. Do not allow the wells to dry out during incubation.

3. Eliminate the residual liquid and fingerprints at the bottom of the plate, otherwise it will affect the OD value.

4. The substrate color development solution should be colorless or very light in color, and the substrate solution that has turned blue cannot be used.

5. Avoid cross-contamination of reagents and specimens to avoid wrong results.

6. Avoid direct exposure to strong light during storage and incubation.

7. After equilibrating to room temperature, open the sealed bag to prevent water droplets from condensing on the cold slats.

8. Any reaction reagent cannot come into contact with the bleaching solvent or the strong gas emitted by the bleaching solvent. Any bleaching component will destroy the biological activity of the reaction reagents in the kit.

9. Expired products cannot be used.

10. If it is possible to spread diseases, all samples should be managed, and the samples and testing devices should be handled according to the prescribed procedures.

Storage Temp. Unopened kit, stored at 4 °C, shelf life 6 months.
Test Range 62.5 pg/mL-2000 pg/mL; Sensitivity: The lowest detection concentration is less than 10 pg/mL.
Shipping Notes
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Exchange/Return Notes
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  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
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SKU: 68066307535

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4.6 ★★★★★
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D. Thomas
Lexington, US
★★★★★ 4
Short-lived but much enjoyed as a chew toy
Color: Consuela the Cactus - Large, Size: Large Dog
I bought two of these for my "granddogs" in the size for Large dogs. The German Shepherd-mix stole the one intended for the border collie-mix and had both toys halfway destroyed within a day--I'm pretty sure they didn't survive much longer after that. Much enjoyed! Maybe I should've bought the Extra Large size, because the Large barely fit in her mouth and she wanted to play tug-of-war, so there wasn't much for me to grab onto. Then the border collie wanted in on the action (she's older, so she needed help tugging!) but there wasn't enough material for her to grab onto without accidentally sinking her teeth into me instead.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 9, 2025
R
Verified Purchase
Ryan Davisson
Draper, US
★★★★★ 5
Best dog toy!
Color: Air Head, Size: Large Dog, Color: Air Head, Size: Large Dog
I got this toy for my dog who loves squeakers not only is it a two in one it actually has three toys inside which I think is amazing!! 10/10 recommend
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 11, 2026
V
Verified Purchase
VPBN
Waukegan, US
★★★★★ 5
Love all the Barkbox toys
Color: Christmas Consuela, Size: Large Dog
This is our third "Consuela"! Our dogs LOVE these toys, and they are the most durable we can find for our heavy chewers. They really like the ball inside as well. Highly, highly recommend these toys. I won't be buying other toys anymore, just barkbox.
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Reviewed in the United States on March 27, 2026
B
Verified Purchase
billy
Dallas, US
★★★★★ 5
My dog is a toy destroyer
Color: Consuela the Cactus - Large, Size: Large Dog, Color: Consuela the Cactus - Large, Size: Large Dog
I’ve had a hard time trying to find toys that my dog, Zara loves because she’s actually very picky. Ive gotten plenty of Kong toys that are well known to be indestructible but she wants nothing to do with them. Then I got her ropes and that seemed to be good for a while until she was getting sick in the middle of the night from eating all of the string. Stuffed toys aren’t good because she’ll rip them within seconds and eat all of the stuffing. I felt like I’ll never score the perfect toy for her. I found the three layered cactus toy and decided to give it a go. It’s clearly designed to be ripped and destroyed to get the ball in the middle which is actually really cute and fun to see all of the layers. The cactus arms have stuffing and of course that’s the first thing she went for and ate. I had to watch her and make sure I took the stuffing away before she ate it. And before I knew it, she ate half of the first layer fabric lol once she got through the first layer, all she’s been doing is walking around the house with the toy in her mouth. But now she’s tired and passed out on the couch from all of the fun. This toy won’t last long with a toy destroyer but it sure does give them something to do and it’s a cute toy. I do recommend!!
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Reviewed in the United States on July 26, 2024
K
Verified Purchase
Klara Gengler
Grantham, US
★★★★★ 5
Last a while. Will be buying more toys soon.
Color: Consuela the Cactus - Large, Size: Large Dog
Best dog toy I have ever bought. I have three dogs, two at the time I bought this toy. It took them over a year to break through the first layer. The flower comes off first so be careful your dog doesn't swallow it. It still has the second layer so it still looks like a cactus. No stuffing to clean up when they tear into it, which makes it far less messy than other toys. One of my dogs is obsessed with fetch so I use it like a ball inside. The oval shape makes it hard to ensure when it bounces that it will bounce straight. It's bounced into walls and other stuff that I wasn't intending it to hit. Not much of a squeak so I can let them play with it for hours and I don't get annoyed with the noise. Great toy that lasts a while.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 25, 2025

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