SKU: 68082240984

Human IFN-γ OneStep OptiQuant ELISA Kit

Sale price$225.00 Regular price$250.00
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Description

Human IFN-γ OneStep OptiQuant ELISA KitProduct Specification Antigen IFN Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Hu Purification Protein A Stability & Storage 2 to 8 C as supplied. Kit Precision Intra assay: 4. 0% Inter assay: 4. 5% Sample type Cell culture supernatant, Serum, Plasma Assay type Sandwich (quantitative) Sensitivity 2. 577pg ml Range 15. 63 pg mL 1000 pg mL Recovery Cell culture supernatant: 93% Serum: 109% Plasma: 111% Assay time 45 minutes

Product Specification


Antigen IFN-γ
Immunogen Recombinant Protein
Antibody Type Recombinant mAb
Reactivity Hu
Purification Protein A
Stability & Storage

2 to 8 °C as supplied.

Kit


Precision Intra-assay: 4.0%;
Inter-assay: 4.5%
Sample type Cell culture supernatant, Serum, Plasma
Assay type Sandwich (quantitative)
Sensitivity 2.577pg/ml
Range 15.63 pg/mL – 1000 pg/mL
Recovery Cell culture supernatant: 93%
Serum: 109%
Plasma: 111%
Assay time 45 minutes
Species reactivity Human
NIBSC convert

The dose response curve of the International Standard (87/586) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (87/586) units, use the following equation: NIBSC (87/586) approximate value (IU/mL) = 0.02 x OneStep Human IFNγ value (pg/mL).

Background

Interferon-gamma (IFN-γ) is a dimeric cytokine central to innate and adaptive immunity, primarily produced by activated T cells and natural killer (NK) cells. Structurally, IFN-γ exists as a homodimer, with each subunit comprising six α-helical domains stabilized by intra- and interchain disulfide bonds. It signals through a heterodimeric receptor complex composed of IFNGR1 and IFNGR2, initiating JAK-STAT pathway activation. Post-translational glycosylation at asparagine residues (e.g., Asn25 and Asn97 in humans) enhances its stability and modulates receptor interaction dynamics. Immune Regulatory Roles and Therapeutic Cross-Talk of IFN-γ: Interferon-gamma (IFN-γ) orchestrates multifaceted immune responses, exerting critical antiviral and antitumor defense by inducing cellular antiviral states and enhancing tumor immunosurveillance through upregulation of MHC class I/II expression. It activates macrophages to bolster phagocytosis, reactive oxygen species production, and pathogen clearance, while polarizing CD4+ T cells into Th1 subsets to amplify cell-mediated immunity. Despite its protective roles, IFN-γ exhibits a dual nature in inflammation, where excessive levels contribute to autoimmune disorders (e.g., rheumatoid arthritis, multiple sclerosis) and chronic inflammatory pathologies. Therapeutically, IFN-γ synergizes with proinflammatory cytokines like TNF-α and IL-12 to amplify immune activation, while counteracting the immunosuppressive effects of IL-4 and IL-10, highlighting its pivotal yet complex role in balancing immune defense and inflammatory regulation. Advantages of this OneStep ELISA Kit Design: Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time. High Efficiency: Achieves results within 45 minutes, ideal for high-throughput studies or urgent diagnostics. Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation. Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable IFN-γ quantification in time-sensitive or resource-limited settings.

Shipping Notes
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Exchange/Return Notes
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SKU: 68082240984

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Christine
Fort Morgan, US
★★★★★ 4
Power Adapter Not Included
Color: Brown +Wireless
I'm initially impressed with this valet tray. I purchased two for my husband: one for his nightstand and one for his dresser, as he likes to keep things organized. We liked the idea of the wireless charging capabilities, but were a little disappointed to discover that the power adapter (wall plug) is not included with the tray. The tray includes a charging pad and a cord, but no wall plug. We don't have an extra pair in the house, so we'll have to wait until a pack arrives before we can test out the charging capabilities. That aside, the trays are a handsome set. I think 20% larger might serve us a tad better, but this will do very nicely for now. We're able to fit almost everything we need in the tray and that will leave the tables looking much tidier, even if one or two items don't quite make it in.
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Reviewed in the United States on October 20, 2025
A
Verified Purchase
A Kisinger
Phoenix, US
★★★★★ 5
Great organization and a charger too!
Color: Blue
Pros: charge pad works well to charge not only phones but watches as well,materials are quality and the blue color is very appealing. Fits well on the night stand and has great sized compartments for items to stay organized. Cons: none that I can note
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Reviewed in the United States on October 10, 2025
A
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Alisha French
San Leandro, US
★★★★★ 5
3 year anniversary is leather
Color: Brown +Wireless
I bought this for our third year wedding anniversary, as a gift for my husband. The 3rd year is supposed to be a gift inspired by leather. The tray is very durable and has lots of compartments. My husband uses it on his nightstand to keep things that he has in his pocket when he empties them the end of the night. The wireless charger is very convenient, although he prefers to actually plug in his phone rather than wirelessly charge it. The charger cable itself is surprisingly of great quality. The leather isn’t too bad either. Good gift for the price point.
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Reviewed in the United States on July 29, 2025
D
Verified Purchase
DSLR user
Waukegan, US
★★★★★ 5
Organizer tray
Color: Brown +Wireless
This thing's style is 1960's cool.. Great organizer and charger.
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Reviewed in the United States on January 24, 2026
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Danny Wilcoxx
Whiting, US
★★★★★ 5
Cute Tray, Bad Charger
Color: Black
Worked for about a month, now it will only charge sometimes. Edit: Seller contacted me and replaced item. It is working perfectly fine now.
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Reviewed in the United States on March 28, 2025

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