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Description
mIHC Dye (TSA540)Description mIHC Dye (TSA540) is a high performance fluorescent dye designed for multiplex immunohistochemistry (mIHC) based on tyramide signal amplification (TSA) technology. It enables robust, covalent fluorescent labeling on formalin fixed paraffin embedded (FFPE) tissues, frozen sections, and tissue microarray (TMA) slides, supporting multi target, in situ biomarker detection in a single tissue section. This reagent provides strong, stable
Description
mIHC Dye (TSA540) is a high-performance fluorescent dye designed for multiplex immunohistochemistry (mIHC) based on tyramide signal amplification (TSA) technology. It enables robust, covalent fluorescent labeling on formalin-fixed paraffin-embedded (FFPE) tissues, frozen sections, and tissue microarray (TMA) slides, supporting multi-target, in situ biomarker detection in a single tissue section.
This reagent provides strong, stable fluorescence with Ex/Em: 520 nm / 540 nm, compatible with standard fluorescence microscopes and multispectral imaging systems. It delivers greatly amplified signals compared to conventional immunofluorescence, ideal for low-abundance antigens and spatial profiling of the tissue microenvironment.
Key Features
TSA-based signal amplification: Ensures high sensitivity and high signal-to-noise ratio, suitable for detecting low-abundance antigens.
Covalent binding: Binds to tyrosine residues covalently, maintaining excellent stability during antibody stripping and repeated staining cycles.
Broad Compatibility: Applicable to FFPE tissues, frozen sections, and TMA slides, meeting diverse experimental needs.
Spectral Characteristics: 540 nm spectral channel, matching AF514, Opal 540, and similar orange-yellow fluorophores.
Easy to Use: Ready-to-use formulation with included signal amplification reaction buffer, no complex pre-processing required.
Application Scope: For research use only; not for in vitro clinical diagnosis or human trials.
Principle
TSA540 adopts horseradish peroxidase (HRP)-conjugated secondary antibodies to activate fluorescently labeled tyramide substrates. The activated tyramide rapidly forms covalent bonds with tyrosine residues near the antigen-antibody complex, forming stable fluorescent deposits. After that, the antibodies can be stripped by heat repair without removing the dye signal, allowing sequential labeling of multiple targets on the same tissue section to achieve multiplex staining.
Storage & Stability
Storage Condition: Store protected from light at 2–8 °C; avoid direct exposure to strong light.
Shelf Life: Stable for 12 months from the date of receipt when stored according to the required conditions.
Composition
TSA Monochromatic Fluorescent Dye 540, Signal Amplification Reaction Buffer
Application
Mainly used for scientific research of immunohistochemical staining of tissues, paraffin sections, and TMA chips; not for in vitro clinical diagnosis or human trials.
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4.0 ★★★★★
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Product Reviews
★★★★★ 5
Whipped and Smooth
Color: Tan
I didn’t know if I was going to love this as much as the drops, but this has an immediate blurring effect and it is whipped. So far I LOVE IT! My skin feels smooth with light to medium coverage. I love that it’s sunscreen.
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Reviewed in the United States on August 26, 2025
★★★★★ 4
Excellent product
Color: Medium
Excellent product, really works & looks good
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Reviewed in the United States on April 16, 2026
★★★★★ 5
Beautiful sunscreen
Color: Medium
The perfect skincare/sunscreen/tint! I am a huge fan of tinted moisturizers and struggle with finding the correct color, texture and staying power. Color science is a clear winner here. A luxurious product, creamy, perfect moisture, coverage and the perfect tone. A++ will buy again…..and again.❤️
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Reviewed in the United States on September 3, 2024
★★★★★ 3
I love this product
Color: Medium
I love this product. Best tinted moisturizer I've ever used. My low star rating is for the product container. On this tube (and the last one I ordered as well) the top separates from the base. When this happens, I can't pump the product out. I have to fiddle around with it quite a bit to get it reattached and then fiddle around some more to get it pumping again. At the price of this product, this issue should really be addressed. I can't believe I'm the only one who is dealing with this. Especially since the last two tubes performed in the same way.
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Reviewed in the United States on March 5, 2017
★★★★★ 5
SO GOOD
Color: Light
This product is so velvety and goes onto the skin so nicely. As per usual, it feels like natural skin but it has great coverage -- I was pleasantly surprised. I don't know what Colorscience does, but they have the magic. I am a forever customer of all of their products. It's a plus that their products are good for the skin AND have SPF as well.
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Reviewed in the United States on January 8, 2024