Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 16 - Aug 21
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human CPT1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against carnitine palmitoyltransferase I (CPT1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of carnitine palmitoyltransferase I (CPT1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Carnitine palmitoyltransferase I | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Carnitine palmitoyltransferase 1 (CPT1), also known as carnitine acyltransferase I, CPTI, CAT1, CoA:carnitine acyltransferase (CCAT), or palmitoyl-CoA transferase I, is a mitochondrial enzyme responsible for the formation of acylcarnitines by catalyzing the transfer of the acyl group of a long-chain fatty acyl-CoA from coenzyme A to l-carnitine. The product is typically palmitoylcarnitine, but other fatty acids may also be substrates. It is part of a family of enzymes known as carnitine acyltransferases. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.5 ★★★★★
Based on 20 reviews
Sort
Product Reviews
★★★★★ 4
Great portable second screen
Size: 15.6" 1080P
Really convenient for a second screen on the go. The display looks sharp and it’s lightweight enough to carry around easily.
Setup is simple, just plug and play. Not super bright, but works well for most uses.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 10, 2026
★★★★★ 5
Exceptional - Better than one twice the price
Size: 15.6" 1080P
I bought a different external monitor a few years ago for twice the price and it was abominable compared to this monitor. This monitor is absolutely perfect. The speakers are definitely tinny like others mentioned, so don't expect to DJ a party with this thing, but for work it's perfect since that means voices are clearer anyways. The sound just isn't as full/rounded with proper bass, but it doesn't even matter because there's also a headphone jack on the monitor, too. USB-C is a total game changer for powering and passing full audio-visual in a single port. The included cables are also a big win since getting a spare data USB-C cable can get pricey and I know the one included is guaranteed to work with this monitor. It was literally plug-and-play with my 2023 16" Macbook Pro. I could go on and on about how every detail of this monitor is perfect, like how the stand is also a screen protector cover and how light and easy it is to carry and how the little grippy feet hold it in place confidently, but my point is THIS is FINALLY the external monitor I've been looking for!!!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 26, 2026
★★★★★ 5
Great portable display for the price.
Size: 15.6" 1080P
This monitor works very well. I bought because I needed an extra monitor for my iPad on my work desk. But I don’t have an outlet at this desk. The iPad powers the monitor just fine, though it drains the battery pretty quickly. I ended up also buying a 20ft USB-C cable and running that to the nearest outlet to power the monitor and my iPad. I also have a little phone holder standing the display up.
The quality is pretty good for the price point. Definitely not my highest quality screen. It's perfect for spreadsheets and the colors are very passable anyway.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 21, 2026
★★★★★ 5
Really good.
Size: 15.6" 1080P
Perfect size. Love the cover that comes with it. Easy storage and portability. Perfect to work on the move. The screen itself looks really good for the price. I connected with the type c and turn on and off as my computer does. Amazing and easy to connect. Doesn't flickers.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 2, 2026
★★★★★ 4
Overall great value, for the stated purpose of being a portable monitor
Size: 15.6" 1080P
This is a review of two monitors:
MNN Portable Monitor 15.6inch FHD 1080P (no model number listed) M156F01 is marked on the box
Arzopa Portable Monitor, 15.6'' 1080P model number: “S1 Table”
I ordered the Arzopa monitor first, as kind of an impulse buy. I was going to be traveling and wanted to take my mini Ryzen 9 PC with me, but it had no monitor. I found the Arzopa monitor had good reviews and the price was right so I ordered. Then later I discovered it was a MATTE panel. I’m not sure how I missed that earlier, but I would NOT have ordered it if I knew that it was matte.
Matte finishes on monitors are supposed to be easier on eyes as the matte surface supposedly reduces glare. But in my experience, there is a problem with this feature. First, any monitor positioned properly should never have a problem with glare. For example, you don’t position a monitor facing a large window or another source of strong light. If your monitor is positioned correctly, glare should not be a possible problem to be solved. Second, the matte finish on most monitors I have seen tends to greatly distort the picture quality. Imagine you are standing outside at noon, but the area you are in is foggy. The view you have of your surroundings (through a light fog) is similar to the picture quality of any matte finish monitor. At least, in my experience.
I was sitting in front of my two large (not portable) monitors, thinking about the mistake I made in ordering a matte finish portable monitor. And I realized…I was so used to using dual monitors that I would really want two monitors for travel, also.
I read some reviews of the MNN monitor and someone described it as a GLOSSY panel. Perfect. I decided to order the MNN to use as my “primary” travel monitor with the Arzopa to be used as my “second” monitor for a dual monitor setup while traveling. That led to mistake number 2. They are both matte finish. When I realized this, they had both shipped. And they were delivered very quickly , both arriving on the same day.
I decided to give these monitors a try, and I’m glad I did. While they are both described officially as “matte”, the picture qualify is closer to what I would expect from a true “glossy” surface computer monitor. And they both have much more than acceptable image quality.
So to compare them:
FEATURES:
Their features are identical other than their physical controls. For adjusting these monitors, there is a difference between them, and I PREFER one over the other, as far as controls go.
Arzopa has multi-function push buttons on the left to adjust settings like brightness/contrast.
MNN has push-buttons and a teeny tiny joystick on the right to adjust settings like brightness/contrast. I think this is an odd design decision for a -portable- monitor.
The Arzopa controls are both more intuitive and (I believe) probably more sturdy / more likely to be reliable over time. We’ll see if the tiny joystick of the MNN survives a few trips bouncing around in my checked bags.
DISPLAY QUALITY
I was very pleasantly surprised by the image quality of both of these monitors. I would rate them both a solid 9/10 on image quality. I’ve seen better, but only with OLED panels. Between the two if I had to pick a winner for image quality it would be the MNN. But the difference is so slight. I think they both deserve a “9”. While I can tell they are both matte (and I do not like that) the matte finish of these monitors is not as “foggy” as most matte monitors. It’s not matte enough to detract from the overall image quality, which is great.
The factory settings of both monitors had the brightness (black lefel) set too high. Brightness is what controls how “black” black items will appear. If the Brightness is set too high, then something which should be color black will appear to be gray or (worse) even light gray. After I turned down the brightness a bit on both monitors, the black level of both was very satisfying.
Speakers: I would never try to use speakers on a portable monitor, so I can’t comment on this. I don’t think the Arzopa even has speakers, but it doesn’t matter, as I would not use those.
STAND:
They have virtually identical magnetically attached pleather stands that can be used to support the monitors in vertical or horizontal orientation. The magnets on both are pretty weak. However, it should be adequate for use on a solid desk or table. You might not be happy on an airplane if you hit turbulence at all.
Summary and suggestion for improvement:
I would give both of these a 4-star rating on the Amazon scale. To earn a 5-star rating I would suggest:
1) Replace the magnetic stand with something that attaches to the monitor more firmly.
2) Include a USB-C cable with a right-angle connector on one end. For dual monitor use, these both have cables sticking out the left side. If you want to set them side by side, you need to keep them about 2” apart, because the cables interfere with setting them close together, like you want to. The most obvious solution would be a right-angle USB-C cable. I have ordered one. But it would cost nothing for Arzopa and MNN to include this.
3) Make the above changes without increasing the price. If you could do that, I would rate these monitors a solid 5/5. There are better monitors for sale. But for the stated purpose and price? These are close to what I would want. Very close.
FINAL THOUGHT: (Which One Should I Buy?)
There are many portable monitors available with plenty of options in the same relative price range. The two I am reviewing here are a couple of the most highly rated. If you are trying to choose between these two specific monitors, I would recommend you buy the Arzopa. While the MNN has slightly better image quality, they both have really good image quality. I think the design of the Arzopa is just a bit more appropriate for the stated purpose of being portable. If you are leaning toward the MNN however, I would not discourage you from buying it. If you’ve narrowed down your decision to these two specific monitors, I don’t think you can possibly choose “wrong” if you buy only one of them. They both exceeded my expectations.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 16, 2025