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Description
RNase RProduct Specification Synonyms Ribonuclease RRNase R Amino Acid Sequence Expression System E. coli Molecular Weight 94 kD Purity 95% by SDS PAGE Conjugation Unconjugated Tag His Tag Physical Appearance Liquid Storage Buffer 50mM Tris HCl, 200mM NaCl, 1mM DTT, 0. 1mM EDTA, 50% (v v) Glycerol, 0. 1% (w v) Triton X 100 (pH7. 5 @25) Stability & Storage Store at 25 ~ 15 for 1 years Reference [1] Cheng, Z. F. Purification and Characterization of the
Product Specification
| Synonyms | Ribonuclease R、RNase R |
| Amino Acid Sequence | |
| Expression System | E.coli |
| Molecular Weight | 94 kD |
| Purity | ＞95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50mM Tris-HCl, 200mM NaCl, 1mM DTT, 0.1mM EDTA, 50% (v/v) Glycerol, 0.1% (w/v) Triton X-100 (pH7.5 @25℃) |
| Stability & Storage | Store at -25 ~ -15℃ for 1 years |
| Reference | [1] Cheng, Z.-F. Purification and Characterization of the Escherichia coli Exoribonuclease RNase R COMPARISON WITH RNase II[J]. Journal of Biological Chemistry, 2002, 277(24):21624. |
Background
Ribonuclease R (RNase R) is a Mg2+ dependent 3'→5' exonuclease derived from Escherichia coli. RNase R can digest all linear RNA. However, it cannot digest ring-shaped RNA, lasso structure or double-stranded RNA molecules with 3 'end protruding ends less than 7 nucleotides, tRNA and 5SRNA with complex secondary structure. RNase R is commonly used in gene expression and variable shear studies and can digest linear RNA to enrich circular RNA or lasso structured RNA. This product does not contain DNase, other RNA endonuclease and exonuclease activities.
Components
Storage Solution: 20U/μl RNase R, 50mM Tris-HCl, 200mM NaCl, 1mM DTT, 0.1mM EDTA, 50% (v/v) Glycerol, 0.1% (w/v) Triton X-100 (pH7.5 @25℃) 10*Reaction Buffer: 200mM Tris-HCl, 1M KCl, 1mM MgCl2 (pH8.0 @25℃)
Protocol
Set up the following reaction in a microcentrifuge tube on ice. Add the following components in sequence. 2）Reaction at 37℃ for 10 min-30 min
Guidelines
1. The activity of RNase R requires 0.1-1.0 mM Mg2+;
2. With the increase of substrate RNA, digestion time and enzyme amount can be appropriately prolonged
Unit Definition
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