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Description
Rat CASP-3 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Gently wash adherent cells with pre-chilled PBS, then trypsinize and collect the cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with pre-chilled PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Collect the supernatant for analysis, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Caspase 3 (CASP-3) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Caspase 3 (CASP-3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Caspase 3 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Caspase-3 (Caspase-3) is a caspase protein that interacts with caspase-8 and caspase-9. It is encoded by the CASP3 gene. CASP3 homologs have been discovered in many mammalian species with complete genomic data. CASP3 is a member of the cysteine-aspartate protease (caspase) family. The sequential activation of caspases plays a central role in the execution phase of apoptosis. It exists as an inactive proenzyme. Proteolytic processing at a conserved aspartate residue produces two subunits, a large and a small, which dimerize to form the active enzyme. This protein cleaves and activates caspases 6 and 7, which are themselves processed and activated by caspases 8, 9, and 10. It is the primary caspase involved in cleaving the amyloid β-4A precursor protein. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids |
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4.3 ★★★★★
Based on 6 reviews
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Product Reviews
★★★★★ 4
Overall, a really solid choice
Color: Light Blue, Size: 6
This suit is a great option for a summer wedding or any formal event! The linen fabric keeps it lightweight and comfortable, which is perfect for kids, and the overall look is really sharp and stylish. The double-breasted jacket adds a nice elevated touch, and the full 5-piece set makes it easy to have a complete outfit without extra shopping.
Everything fit well and looked great for the occasion. My only small complaint is the tie, it’s fine, but I do wish it was a bit nicer to match the quality of the rest of the suit.
Overall, a really solid choice that looks adorable dressed up, with just a minor detail that could be improved.
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Reviewed in the United States on April 23, 2026
★★★★★ 5
TAMIFLY Boys Linen Suit 5 Piece Summer Wedding Suits
Color: Light Grey, Size: 14
I am very impressed with this 5-piece boys linen suit set. It includes everything needed for a complete formal outfit—jacket, pants, dress shirt, tie, and bow tie—which makes it a very convenient all-in-one option for special occasions.
The linen fabric feels lightweight and breathable, which is especially important for kids who may be wearing a suit for long periods of time. Even in warmer weather, the material helps keep things comfortable without feeling heavy or restrictive.
The suit jacket is well designed with thoughtful tailoring details like a peak lapel, double-breasted closure, and functional pockets. It gives a polished, structured look while still being suitable for children. The lining also adds to the overall comfort and quality feel.
The pants are equally practical, featuring an adjustable waistband that helps ensure a better fit as kids grow or move around. The slanted pockets and smooth zipper closure make them easy to wear while maintaining a clean, formal appearance.
One of the biggest advantages of this set is its versatility. It works for a wide range of occasions such as weddings, baptisms, church events, graduations, parties, performances, and other formal or semi-formal gatherings. Having both a tie and bow tie included also allows for different styling options depending on the event.
The sizing guidance is also helpful, especially the recommendation to size up for fast-growing children. The suit maintains a structured look while still allowing enough comfort for movement.
Overall, this is a stylish, complete, and practical formal suit set for boys. I would definitely recommend it for parents looking for a well-rounded outfit for special occasions that combines elegance, comfort, and convenience. The tuxedo is quite expensive.
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Reviewed in the United States on April 19, 2026
★★★★★ 5
Sharp Little Guy Alert!
Color: Navy, Size: 7, Color: Navy, Size: 7
I received this navy boys linen suit set in size 7 and it is absolutely adorable — and impressively well put together for the price! The navy color is rich and deep, not washed out or cheap looking, and the double breasted jacket with peak lapels gives it a really distinguished, grown-up look that is still perfectly sized and proportioned for a little boy. My son looked like a million bucks.
The five piece set is a great value — you get the jacket, pants, dress shirt, tie, and bow tie all in one purchase, which takes a lot of the guesswork out of pulling together a complete outfit. Everything coordinates beautifully right out of the box. The jacket details are lovely — the welt chest pocket, the lined interior, the buttoned cuffs — it all feels intentional and well-designed rather than thrown together.
The fabric has a nice linen-blend texture that looks elevated and holds up well — I was pleasantly surprised by how little wrinkling occurred even after hours of wear, which is not always the case with linen blends. The construction feels durable and sturdy, like it will survive more than just one special occasion before outgrowing it. The adjustable waistband on the pants is a thoughtful touch for growing kids.
On sizing — read the size chart carefully since it reflects suit measurements rather than body measurements. If your child is between sizes or growing fast, sizing up is the smart move. We ordered a size 7 and the fit was right on target.
Dry clean only, so keep that in mind for care. For a special occasion suit at this price point, the value is exceptional — you would easily pay two or three times this at a department store for comparable quality. Highly recommend!
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Reviewed in the United States on April 10, 2026
★★★★★ 5
True to size, with hidden adjustable waistband
Color: Light Grey, Size: 10, Color: Light Grey, Size: 10
This is SO adorable!
What I liked:
-It fits true to size, and the pants are adjustable. My son is 9, so I bought him 10Y. It’s slightly large on him, as expected. However, it doesn’t look too large. The pants have elastic inside the waist on both sides—the kind which allows you to pull on it to select how tight you want them, then secure the elastic to a hidden button. It also has belt loops, but I was able to tighten the pants enough that they weren’t needed.
-The pants have pockets.
-The material is soft and looks of high quality. The inner lining of the jacket feels soft & smooth against the skin, and there’s a small pocket inside the jacket.
-The tie is brilliant! It comes pre-tied and on an elastic bungee. The elastic hides perfectly under the collar. The bow tie is also adjustable.
There wasn’t really anything I didn’t like—Perhaps that the white shirt is thin. However, since I plan to have him wear it under the suit coat, it’s not an issue.
When my son put this on, I couldn’t stop saying “aww!” In all, I’m impressed.
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Reviewed in the United States on April 9, 2026
★★★★★ 5
Crisp, Classic, and Perfect for Summer Events
Color: Navy, Size: 14, Color: Navy, Size: 14
This boys’ linen suit set arrives neatly packaged and looks polished right out of the bag. The white shirt, blue vest or jacket, and coordinating accessories create a clean, formal look that works beautifully for weddings, photos, and other warm‑weather events. The fabric feels lightweight and breathable, which is exactly what you want in a summer suit.
The double‑breasted jacket adds a stylish, elevated touch without feeling too heavy, and the pants have a comfortable fit that gives kids room to move. The included bow tie and necktie make it easy to switch between a more traditional or modern look, and the overall sizing appears accurate for a formal set.
As a full five‑piece outfit, this is a convenient, ready‑to‑wear option for parents who want something sharp and cohesive. It photographs well, feels comfortable for kids, and offers great value for a complete formal ensemble. A solid pick for ring bearers, family photos, or any special occasion.
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Reviewed in the United States on April 7, 2026