SKU: 81853634746

Gastric Organoid Cytokine Set, Mouse

Sale price$313.20 Regular price$348.00
Save 10%

Pay in installments of $87.00 with ShopPay, AfterPay and Klarna

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Sep 12 - Sep 17

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

Gastric Organoid Cytokine Set, MouseProduct Specification Species Mouse Endotoxin <0. 1EU g Physical Appearance Lyophilized powder Reconstitution Reconstitute at 0. 1 1 mg ml according to the size in ultrapure water after rapid centrifugation. Stability & Storage 12 months from date of receipt, lyophilized powder stored at 20 to 80. 3 months, 20 to 80 under sterile conditions after reconstitution. 1 week, 2 to 8 under sterile conditions after reconstitution. Please avoid repeated freeze

Product Specification


Species Mouse
Endotoxin <0.1EU/μg
Physical Appearance Lyophilized powder
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

Stability & Storage

·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.

Components

Protein

Reference dosage

100 ml system

1000 ml system

cytokine 1

100ng/ml

10μg

100μg

cytokine 2

100ng/ml

10μg

100μg

cytokine 3

50ng/ml

5μg

50μg

cytokine 4

100ng/ml

10μg

100μg

cytokine 5

100ng/ml

10μg

100μg

cytokine 6

100ng/ml

10μg

100μg


Protocol

Preparation of Complete Culture Medium for Mouse Gastric Organoids: Mix the basal culture medium, cytokines (1-6), and various additives in the predetermined proportions to prepare the complete culture medium.

1. Primary Culture
- (1) In the laminar flow cabinet, quickly open the abdominal cavity of the mouse, remove the stomach tissue, and place it in pre - cooled PBS (with penicillin, streptomycin, and primary antibiotics). Open the stomach, rinse the contents, and remove the outer layer of fat and blood vessels.
- (2) Collect the cleaned tissue blocks into a 1.5ml EP tube. Use a sterile fine - tipped ophthalmic surgical scissors to mince the tissue blocks as finely as possible (into a homogenate). The degree of tissue mincing is related to the digestion time. The finer the mincing, the shorter the digestion time. Transfer the minced tissue to a 15ml sterile centrifuge tube.
- (3) Add 10 times the volume of tissue digestion solution for digestion. Incubate at 37℃ with shaking for 10 - 30min. Observe the situation at any time during the digestion process. When a large number of cells are observed to leak out in the field of view and most of the cells are in the form of cell clusters, the digestion can be terminated (note: do not digest to the single - cell state).
- (4) In the tissue suspension confirmed to be digested, add fetal bovine serum (FBS) to a final concentration of 2 - 5% or bovine serum albumin (BSA) to a final concentration of 0.1%, and mix by pipetting.
- (5) Pass the tissue and cell suspension through a 70µm cell strainer. Centrifuge the filtered cell suspension at 1000rpm for 5min. After centrifugation, discard the supernatant.
- (6) If obvious red precipitate (red blood cells) is observed in the precipitate after centrifugation, add 2ml of red blood cell lysis solution, mix by pipetting, and leave it for 3min. Centrifuge at 1000rpm for 5min, and then discard the supernatant.
- (7) Resuspend the precipitate with an appropriate amount of basic culture medium or PBS.
- (8) Mix the matrix gel and crypts in a suitable proportion. For example, for a 24 - well cell culture plate, spot 25 - 30ul of the matrix gel mixture into each well for coating (operate at 4℃).
- (9) Place the coated culture plate in a 37℃ incubator for 20 - 30min to gel. Add an appropriate amount of mouse stomach organoid complete culture medium (at room temperature) for culture.

2. Organoid Passaging Culture
- (1) Aspirate the culture medium with a pipette, add 1 - 2ml of 4℃ PBS to each well, and leave it for 2min.
- (2) Gently pipette the matrix gel to collect it in a 15ml centrifuge tube. Adjust the volume with PBS to 10 - 14ml, and leave it at 4℃ for 20 - 30min. Centrifuge at 1000rpm for 5min to discard the liquid and retain the precipitate (group every 3 - 5 wells).
- (3) Resuspend the organoids with an appropriate amount of matrix gel, and spot 25 - 30ul of matrix gel into each well of a 24 - well cell culture plate. Place it in the incubator for 20 - 30min to gel, and then add an appropriate amount of mouse stomach organoid complete culture medium.

3. Organoid Cryopreservation
- (1) Aspirate the culture medium with a pipette, add 1 - 2ml of 4℃ PBS to each well, and leave it for 2min.
- (2) Gently pipette the matrix gel to collect it in a 15ml centrifuge tube. Adjust the volume with PBS to 10 - 14ml, and leave it at 4℃ for 20 - 30min. Centrifuge at 1000rpm for 5min to discard the liquid and retain the precipitate (group every 3 - 5 wells).
- (3) Add an appropriate amount of organoid cryopreservation solution, and gently pipette to resuspend. For example, for a 24 - well cell culture plate, cryopreserve 2 - 3 wells in one tube, with a volume of 1ml per tube.
- (4) Label the information properly, and then transfer it to liquid nitrogen for long - term storage after programmed cooling.

4. Organoid Thawing
- (1) Take 10ml of DMEM/F12 basic culture medium in a 15ml centrifuge tube.
- (2) Remove the cryopreserved organoids from the liquid nitrogen tank, and quickly place them in a 37℃ water bath to thaw.
- (3) During the water bath thawing process, gently shake the cryovial to ensure that the cryopreservation solution is completely thawed within 1 - 2min.
- (4) Quickly transfer the thawed organoids to a 15ml centrifuge tube, gently pipette 6 - 8 times with a pipette, centrifuge at 1000rpm for 5min, and then remove the supernatant to collect the organoid precipitate.
- (5) Resuspend with matrix gel, spot 25 - 30ul of matrix gel into each well of a 24 - well cell culture plate, place it in the incubator for 20 - 30min to gel, and then add an appropriate amount of mouse stomach organoid complete culture medium.

Guidelines

Avoid vortex oscillation; Store in aliquots to reduce the number of freeze-thaw cycles

Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 81853634746

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.6 ★★★★★
Based on 23 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
N
Verified Purchase
NV46
San Leandro, US
★★★★★ 5
good price and nice design for easy use
Color: A-silver
works well and perfect for our bamboo cleaning brushes
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 18, 2026
B
Verified Purchase
B.Barri
Louisville, US
★★★★★ 3
Leans forward with suction cups
Color: A-silver, Color: A-silver
I really like all of the spaces and the metal is good quality. However it leans forward when using the suction cups. I haven’t had that issue with other holders.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 5, 2026
K
Verified Purchase
24karats
New York, US
★★★★★ 5
Inexpensive organizational tool
Color: A-silver
What do you do with your wet kitchen sponge? You can't store it in a drawer or cabinet because it's wet, and you need to keep it handy because it's your primary kitchen cleaning tool. This caddy offers a practical, low-cost solution. I like the quick, non-permanent suction cup installation as it allows for easy removal if I need to access the entire sink. I may eventually use the adhesive strip once I find the exact location that works best for me, and it's nice to have both options. I also like the additional brackets for the sink strainer and scrubbing brush. Overall, I think this caddy is a very handy and inexpensive solution for managing the inevitable kitchen sink clutter.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 10, 2026
C
Verified Purchase
Constance A. Holden
Belleville, US
★★★★★ 5
Reinvented the wheel
Color: A-silver
Top quality item. The stainless steel is sturdy and attractive. I like the extra bar at the front for drying your dishcloth. Better mounting system.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 20, 2026
T
Verified Purchase
Tarra
New York, US
★★★★★ 5
great shelves
Color: C. White - Modern Minimalist Style, Number Of Shelves: 3, Size: 36inches
These are a a great set of floating shelves! They were easy to install, feel sturdy, and look clean and modern on the wall. The set of three is perfect for displaying decor, photos, plants or small storage items while adding extra space without taking up room. Great quality, stylish, and a simple way to upgrade any space. They work perfect for my figurines to be displayed.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 19, 2026

recommand products