SKU: 84930630449

Human IL-1β High Sensitivity ELISA Kit

Sale price$202.50 Regular price$225.00
Save 10%

Pay in installments of $56.25 with ShopPay, AfterPay and Klarna

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Sep 7 - Sep 12

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

Human IL-1β High Sensitivity ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti human il 1 Capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The il 1&beta in the sample; Binding to solid phase antibodies.

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti-human il-1β Capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The il-1&beta in the sample; Binding to solid-phase antibodies. After thorough washing to remove free and unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color reaction and il-1&beta in samples; There is a positive correlation between the concentration of. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: Pre coated 96 well plates, standards, il-1β One copy of detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.02pg/ml

Detection Range: 0.19-12 pg/ml

Recovery Range: 93-112%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 1 (IL-1) is a 17kDa extracellular polypeptide, which is divided into il-1α and il-1β two proteins. Il-1β is a protein precursor secreted by activated macrophages, which is secreted by caspase1 (casp1/ice)The activated form produced by hydrolysis. This cytokine is an important mediator of the inflammatory response and is involved in many cellular activities, including cell proliferation, differentiation and apoptosis. IL-1 is an important family of proteins, which are derived from bioactive monocytes and participate in inflammatory and immune responses. Il-1β Elevated expression can produce a variety of different autoinflammatory syndromes, the most significant of which is the monogenic condition known as caps, which is due to the triggering of il-1β It is caused by mutations in the processed inflammasome receptor NLRP3. This kit is used to detect human il-1β Of the mature form and the uncleaved precursor form.

Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 84930630449

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.6 ★★★★★
Based on 21 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
D
Verified Purchase
Dawn
Boise, US
★★★★★ 5
Excellent mineral sunscreen with minimal white cast.
Style: SPF 50
This is one of the healthier sunscreens I’ve found so far. You’re not likely going to find a mineral sunscreen that doesn’t leave some white cast. However, this one rubs in pretty good with minimal cast. You can dab on and rub in fairly easily… as long as you don’t use too much the white cast should be minimal. I am not sure how well this would work on darker skin tones. My arms are a medium skin tone… my legs are more of a light-medium tone. I prefer mineral sunscreens, and this is the best one I found so far and compatible to Supergoop mineral play. If you liked that one, you would likely enjoy this Coola one too.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 25, 2025
K
Verified Purchase
Kaitlyn Jacobson
San Leandro, US
★★★★★ 1
Would not recommend
Style: SPF 50
I literally never write reviews but felt compelled to write one for this sunscreen because I was very caught off guard by all the positive reviews. This sunscreen leaves the most insane white sheen that never soaks in and gets on EVERYTHING. I put this on and drove to the beach and had sunscreen on my car seat, the steering wheel, the center console, the door, EVERYWHERE! It took forever to clean up and was such a hassle and that was after having let the sunscreen soak into my skin for an hour. Also the sunscreen is really thick and difficult to spread and makes you look ghostly white. Plus, the sunscreen has a really odd odor that reminded me of play dough? That might not be a negative thing for everyone but I found it unpleasant. There are definitely better mineral sunscreens out there. For the price I expected a much better product.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 11, 2025
J
Verified Purchase
John Reid
Fort Morgan, US
★★★★★ 3
Sticky and white
Style: SPF 50
The sunscreen has a very sticky and thick texture. It will keep you from getting sunburnt, but it will also leave you with a white color.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 22, 2024
B
Verified Purchase
Brona Buys
Charlottesville, US
★★★★★ 4
Good sunscreen
Style: SPF 50
Super good coverage and it lasts a long time in the sun and even through water activities. The only negative is the white cast. Not as bad as others but just be aware it doesn’t fully rub in.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 1, 2025
B
Verified Purchase
Becky
Carnegie, US
★★★★★ 5
Great sunscreen & works for kids - even used in resorts!
Style: SPF 50
Terrific! I don’t feel it is as good as chemical ones but my favorite non-chemical one. Used on a long vacation and it did the job! Also didn’t cause pimples like other thick versions of this.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 9, 2025

recommand products