Mouse IL-1β ELISA Kit(Plus)

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti mouse il-1&beta  Capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process; Il-1&beta  Bound to solid-phase antibody and detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response and sample   Il-1β There is a positive correlation between the concentration of. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: pre coated 96 well plates, standards, il-1β One copy of detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions

Sensitivity: 1.45 pg/ml

Detection Range: 23.44-1500pg/ml

Recovery Range: 83-109%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 1 (IL-1) is a 17 kDa extracellular polypeptide, which is divided into il-1α and il-1β two proteins. Il-1β is a protein precursor secreted by activated macrophages, through caspase 1 (casp1/ice)The activated form produced by hydrolysis. This cytokine is an important mediator of the inflammatory response and is involved in many cellular activities, including cell proliferation, differentiation and apoptosis. IL-1 is an important family of proteins, which are derived from bioactive monocytes and participate in inflammatory and immune responses.

IL-1β Elevated expression can produce a variety of different autoinflammatory syndromes, the most significant of which is the monogenic condition known as caps, which is due to the triggering of il-1β It is caused by mutations in the processed inflammasome receptor NLRP3.