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Description
Mouse SLC30A6 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Solute Carrier Family 30, Member 6 (SLC30A6) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Solute Carrier Family 30, Member 6 (SLC30A6) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Solute Carrier Family 30, Member 6 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Zinc transporter 6, also known as ZNT6/SLC30A6, is an enzyme encoded by the ZNT6/SLC30A6 gene. This gene encodes a member of the zinc transporter protein family. This protein regulates subcellular zinc levels in the Golgi apparatus and vesicles. Its expression is altered in Alzheimer's disease brain plaques. Diseases associated with SLC30A6 include autosomal dominant spastic paraplegia 4 and zinc-deficiency acrodermatitis enteropathicum. Pathways involved include metal ion SLC transporters and peptide hormone metabolism. An important paralog of this gene is SLC30A5. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.7 ★★★★★
Based on 7 reviews
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Product Reviews
★★★★★ 5
My dogs favorite toy so far in two years
Color: C) 4" 2-Pack (Bacon)
I use this with my medium sized dog. These are large for him but that's what I want so I don't ever have to think about them being too small or a swallow hazard. He has been through A LOT OF TOYS in the last couple years, but nothing, NOTHING has he loved as much as these.
His favorite used to be squeaker balls. Then his favorite was crackle balls. But he will gladly ignore both for this one. I don't know if it's the bacon flavor or what, but he go nuts for it.
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Reviewed in the United States on May 5, 2026
★★★★★ 5
Durable Rubber Bone Toy for Aggressive Chewers
I got this rubber bone chew toy in purple (size large) for my dog, and it's been a solid addition to his toy rotation.
What I Liked:
The bacon flavor is a huge hit. My dog was immediately interested in this toy and goes back to it regularly, which speaks to how appealing the scent is to him. The natural rubber material is tough and has held up well to his aggressive chewing - it's designed for heavy chewers and delivers on that promise. The textured surface is great for dental health. I can see how it helps clean his teeth as he chews, and the grooves are deep enough that you could add dog toothpaste for extra cleaning power. It's a functional toy that serves multiple purposes beyond just entertainment.
The rubber material is softer than hard nylon toys, which gives me peace of mind that it won't damage his teeth during extended chewing sessions. It's still plenty durable but has some give to it that makes it safer. This toy genuinely keeps him busy and engaged. It helps redirect his chewing instincts away from furniture and other household items, and it seems to help with anxiety when I need to leave him alone for a bit. At $8.99 (down from $15.99), this is an excellent value for a durable, functional chew toy.
What Could Be Improved:
While it's marketed for all sizes from small to large breeds, I think it works best for medium to large dogs. Small dogs might find the large size a bit cumbersome. The bacon scent is strong initially, but it will fade over time with use and cleaning. Also, we all know it's a little... interesting looking haha.
Overall:
This is a high-quality, durable chew toy perfect for aggressive chewers. The bacon flavor, dental benefits, and tough construction make it a winner. It keeps dogs engaged, helps maintain dental hygiene, and redirects destructive chewing behaviors. At under $9 (well, it is right now, at least), it's a great value and makes an excellent gift for any dog owner dealing with a heavy chewer. Highly recommended.
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Reviewed in the United States on January 18, 2026
★★★★★ 5
The Purple Power Chew My Dog Thinks Is His Life’s Mission
I bought this purple rubber bone hoping it would survive longer than the usual 10 minutes my dog needs to destroy a “durable” toy. Shockingly, this one is still alive and thriving. Honestly, I think it might outlive me.
The material is tough enough for aggressive chewers, but still bouncy and fun, which means my dog is entertained AND my furniture gets a break. It’s the perfect size for medium to large dogs, and it keeps mine busy like he’s working an important full-time job.
He tosses it, chases it, gnaws on it with total devotion… and somehow it barely shows a scratch. The purple color is a bonus, easy to spot in the yard, and it makes him look very fashionable in his ferocious-chewer era.
If your dog believes every toy is a challenge to conquer, this chew toy just might be the worthy opponent they’ve been waiting for. 💜🐶
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 5, 2025
★★★★★ 3
Not my husky's favorite
Size: Large
No bounce, it's too hard and heavy. My husky only uses it once in a while, it is not his favorite thing to chew on.
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Reviewed in the United States on February 28, 2026
★★★★★ 4
Better Than Most, But Still No Match for My Super Chewer
This is a decent chew. It’s made of a softer rubber material and has little to no odor, which I appreciate. It’s been about a week, and while it’s still technically in one piece, it’s starting to show some cracks and tiny puncture holes.
Now, keep in mind, my 100-lb pit mix Scooby Doo is a full on chew and destroy hound. If it can be chewed, he’ll find a way to shred it to bits. This toy held up longer than a lot of others but still didn’t make the “indestructible” cut in our house. He likes it, but it’s not his top pick, probably why it’s still alive.
Overall, it’s a good toy for the price. Probably not for extreme power chewers like Scoob, but it’ll hold up fine for most dogs and give you a little peace and quiet for a while. Do give it a try, Scooby Doo is a chewer in a class of his own, the fact that this has held up past a one 15 minute session is amazing in itself.
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Reviewed in the United States on November 5, 2025
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