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Description
p27 Kip1 Recombinant Rabbit mAb,PBS Only(SDT-361-324)Product Specification Host Rabbit Antigen p27 Kip1 Synonyms Cyclin dependent kinase inhibitor 1B; Cyclin dependent kinase inhibitor p27; Cdkn1b; KIP 1 Immunogen Synthetic Peptide Location Cytoplasm, Nucleus Accession P46414 Clone Number SDT 361 324 Antibody Type Recombinant mAb Isotype IgG Application WB, IHC P, ICC Reactivity Hu, Ms, Rt Positive Sample Daudi, HeLa, MCF7, SH SY5Y, Jurkat, Raji, NIH 3T3, C6 Purification Protein A Concentration 0. 5 mg
Product Specification
| Host | Rabbit |
| Antigen | p27 Kip1 |
| Synonyms | Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Cdkn1b; KIP-1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P46414 |
| Clone Number | SDT-361-324 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Daudi, HeLa, MCF7, SH-SY5Y, Jurkat, Raji, NIH/3T3, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
p27 KIP1 is a member of the Cyclin Inhibitory Protein/Kinase Inhibitory Protein (CIP/KIP) family of cyclin-dependent kinase (Cdk) inhibitors. It plays roles in all phases of the cell division cycle. Its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes enforces the G1 restriction point. Thus, important cellular functions as the cell division cycle, respiration, RNA processing, translation, and cell adhesion, are under p27 KIP1 regulation. Loss or reduced expression is associated with poor outcome in carcinoma of breast, prostate, GI tract and lung.
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