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Description
Human RBMS1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against RNA-binding motif, single-stranded-interacting protein 1 (RBMS1). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of RNA-binding motif, single-stranded-interacting protein 1 (RBMS1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human RNA-binding motif, single-stranded-interacting protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | RNA-binding motif-single-stranded interacting protein 1 is a protein encoded by the RBMS1 gene. This gene encodes a member of a small family of proteins that bind to single-stranded DNA/RNA. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequences (RNP-CSs), containing conserved motifs, namely RNP1 and RNP2, originally described in RNA-binding proteins and required for DNA binding. These proteins have been implicated in diverse functions such as DNA replication, gene transcription, cell cycle progression, and apoptosis. Multiple transcript variants, resulting from alternative splicing and encoding different isoforms, have been described. A pseudogene has been identified at this locus on chromosome 12. RBMS1 has been shown to interact with the polymerase (DNA guide), α1. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.4 ★★★★★
Based on 10 reviews
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Product Reviews
★★★★★ 5
I don’t often leave reviews, but this exceeded my expectations and I LOVE it!!! I NEEDED it!
Style: MULTIBALM
I don’t review things unless they REALLY “wow” me, rare, lol. My eyes had been watering for about a month from allergies, then last week my eyeballs were literally hurting so badly, which that had never happened to me before. So I got eye drops made with ketotifen (from the Walmart App) and it got rid of the pain in my eyeballs and stopped symptoms immediately, thank goodness. Then, from the constant watering, rubbing, dabbing and wiping my eyes, alll above, below and around my eyes became raw and chapped, burning and painful. I tried everything! Aquaphore, Goldbond, Pili Ani, and a lot of other brand name high quality face washes, moisturizers, masks, creams, oils and nothing was helping and some seemed to make it even worse. I saw this product and after reading the reviews, decided to give this one last shot before going to a Dermatologist. The moment I applied it, I felt INSTANT relief and soothing. I also was surprised to literally feel it tighten the skin! This morning I woke up to use it, day 3, and before I did I noticed my skin was so much better!!!…No longer painful, discolored or swollen & puffy, the skin was still silky soft from applying it last night, and my orbital skin is 97% better!!! The pink/red/purple has gone away, it’s no longer tender/burns to the touch, and you can literally feel it nourishing and tightening your skin. After begging, my husband let me try it on his skin (lol) just to make sure I wasn’t going koo koo. He noticed too! And commented about how soft it is! Yes~Exactly! It makes your skin like SILK! It is a tad oily when you first put it on, but it “settles” quickly into a gentle softness and doubles as a primer before you apply your makeup! It smooths out your pores and makes your skin so smooth. Just this morning was the first time I applied makeup with it and was pleasantly surprised. I have tested it putting it on the top of one hand and nothing on the other, timed an hour and WOWWWWW the difference in undeniable! I personally recommend using it after washing your face before you put anything else on (moisturizer, makeup, etc). As long as you give it 10-15 minutes to let it absorb and settle, you’re good to go with or without makeup! I’m just really glad I found this because that skin is so sensitive and NEEDS this type of care. This product gets a solid 5 stars from me and I’m excited to make it part of my morning and evening routines! : )
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Reviewed in the United States on May 5, 2026
★★★★★ 4
Excellent Formula, but the Product Runs Out Far Too Quickly
Style: MULTIBALM
The balm is genuinely hydrating, smooth, and very convenient to use. It applies beautifully, gives the skin a healthy glow, and works well on dry areas, lips, and the face overall. The texture feels comfortable and nourishing without feeling overly heavy or greasy.
My only major disappointment is the value for the price. Even using a very thin layer across the face once each evening, one stick lasted only about a week. I purchased two of them, and both were exactly the same, so this was clearly not an issue with a defective or partially filled tube.
If you intend to use it regularly over the full face, expect it to run out extremely quickly. I purchased mine during a significant sale, and even at the discounted price I still felt disappointed by how little use I got from it.
Overall, the formula performs well, but the quantity does not justify the regular price for consistent daily use.
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Reviewed in the United States on May 24, 2026
★★★★★ 5
Convenient Hydrating Balm for Quick Touch-Ups
Style: MULTIBALM
Really enjoying this balm stick so far. It’s super convenient to toss in a bag and easy to apply throughout the day whenever my skin feels dry or needs a little glow. It glides on smoothly and gives a hydrated, dewy look without feeling heavy.
I especially like using it on dry areas and around fine lines for a quick refresh. The packaging feels nice quality too. Great skincare item for easy on-the-go use.
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Reviewed in the United States on May 14, 2026
★★★★★ 3
Hydrating moisturizer with pleasant scent
Style: MULTIBALM
Update:
I’ve now used 2 sticks. I won’t be buying again. Although I like how convenient this moisturizer is, the price is too high for the amount you get. Also, going through another stick, I’ve noticed no signs that it helps with fine lines or wrinkles. This product is simply an on the go moisturizer or even a highlighter. But I can use castor oil and Vaseline for that. I had to deduct a star.
So far, this moisturizing stick has been a great and convenient addition to my routine. It glides on smoothly and has a light, pleasant scent. The formula is definitely hydrating and leaves my skin feeling soft.
It does go on a bit oily or greasy at first. If that bothers you, rubbing it in helps, though it doesn't absorb immediately—so expect a bit of shine for a while after application.
As a moisturizer, it works well and I would definitely recommend it for anyone looking for a quick, on-the-go option. I haven’t noticed any additional benefits yet (like long-term improvements in skin texture or tone), but it might be too early to say. I’ll update my review if anything changes with continued use.
I do have a couple of concerns. First, the price—it feels expensive for the amount of product you get. Second, I’ve seen some people compare this to Botox, and I’m not sure why. It’s definitely not a substitute, and expecting those kinds of results just isn’t realistic. Along those lines, the product name—“Wrinkle Bounce”—may be a little misleading. While it’s hydrating and may temporarily plump the skin, it doesn’t deliver dramatic wrinkle-reducing effects.
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Reviewed in the United States on June 2, 2025
★★★★★ 5
Youthful, even skin!
Style: MULTIBALM
I’m loving this stick! I almost sent it back after the first two days but I’m so glad I didn’t. This has really helped me look more refreshed. It’s def helped with shallow lines. Overall it’s made me skin more even. It’s very oily and it sits on your skin so I use it before bed. But I’ve noticed it’s helped balance my skin. I usually get really oily during the day but oddly as oily as this is it keeps my skin from getting oily during the day under my makeup. My makeup looks more even. It’s just overall been great for my skin and it’s made me look younger. Def recommend!
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Reviewed in the United States on May 21, 2026