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Description
Human Gastric Cancer Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) the tissue after sampling must be placed in the pre cooled (2 8 c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information (2) prepare several Petri dishes and add 4 pre cooled primary culture buffer B for standby (3) disinfect the sampling bottle, put the tissue into the Petri dish,
Product Specification
| Usage |
1. Primary (1) the tissue after sampling must be placed in the pre cooled (2-8° c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information (2) prepare several Petri dishes and add 4 ℃ pre cooled primary culture buffer B for standby (3) disinfect the sampling bottle, put the tissue into the Petri dish, wash it with primary culture buffer B for three times, and then cut the tissue into a tissue block with a volume of about 1-3mm3using ophthalmic scissors or scalpel (4) the tissue was digested with human gastric cancer primary tissue digestive solution C, and then it was shaken at 37 ℃ for 10-20min (the digestion condition was observed at any time during the digestion process) (5) take a small amount of liquid and observe it under the microscope. After observing more single cells or cell clusters below 70UM under the microscope, add three times the volume of primary culture buffer B to stop digestion (6) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation (7) Matrigel calculation: after step 6, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate (8) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃) (9) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a gel, and add human gastric cancer organoid medium a (restore room temperature) for culture 2. Organoid subculture (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4  &Nbsp&Nbsp&Nbsp b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4 (4) after organoids were collected, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul human gastric cancer organoid culture medium a 3. Organoid cryopreservation (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid (4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube (5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage 4. Organoid resuscitation (1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube (2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting (3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min (4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g (5) resuspend the Matrigel, lay 25ul of Matrigel per well in a 24 well cell culture plate, place it in an incubator for 10-15min to form a gel, and add 500ul of human gastric cancer organoid culture medium A. |
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| Description | Composition:
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| Storage Temp. | 4° C. 3months; -20° C. 1 year, see label for details |
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4.3 ★★★★★
Based on 18 reviews
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Product Reviews
★★★★★ 1
A treat, not a chewing bone. Lasts only a few minutes.
Short lived - lasts only a few minutes with our Golden's. I have used other products like Natural Pet collagen rolls which last a VERY long time with our voracious chewers.
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Reviewed in the United States on November 28, 2025
★★★★★ 3
Not a happy customer
My Aussie ate the chew stick within 5min. Disappointed it didn't last longer. Won't buy again :(
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Reviewed in the United States on May 27, 2026
★★★★★ 4
Massive treats, no tummy rumbles, but a little pricey
I'm always a little tentative when ordering dog treats online, because you don't always know in advance where they're sourced from, or what weird processing might be done to them, or what weird additives might be thrown in. These are sourced in Mexico, and they seem to be straightforward in terms of ingredients. Phew!
The smell of these is not the best, but if you've fed your dog collagen treats before, I'm sure you know what you're getting into with that. The dogs loved them, for sure. These treats are MASSIVE, so if you have a small or medium sized dog, you might want to cut them into two or more pieces. For my 60ish pound dogs, they were very large but manageable, and the way their eyes lit up when I presented it to them, it was like Christmas. LOL
The only reason I'm docking a star is because these are not long-term chews, at least for my dogs. Yak chews will generally last for a couple of hours for them, which I break up over several days by taking the treat away after a while and giving it back to them the next day. These chews were gone within about 5-10 minutes. (Five minutes for my spazzy dog who acts like treats will disappear if they're not hoovered it into her gullet at breakneck speed, and ten minutes for the other who likes to taste his food on the way down.) At almost $6 a pop, these are pretty expensive for 5-10 minutes of enjoyment, so this isn't going to be a treat I buy for them on the regular.
There were no issues with tummy rumbles or nausea or diarrhea afterward, so that's good. And I like getting a little more collagen in these kids because they're both getting up there in age, so their hips and joints can use a little extra love. If you've got the extra money to spring for these, or if your dog paces him- or herself reasonably with dog treats, this could be a good choice for you.
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Reviewed in the United States on September 8, 2025
★★★★★ 5
My non-chewer loves these
He is not into chewing, tried many different ones.
But he’ll chomp down on theses, and they seem to be all good stuff
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Reviewed in the United States on December 17, 2025
★★★★★ 5
Dog loves them, no rawhide!
My dog is obsessed with these! We have a 70 pound pit mix who is a power chewer and these rolls last forever. He goes crazy for the peanut butter taste and it takes him a couple of days to really destroy one entirely. They are very effective for keeping him busy as the size is massive. I like giving him something so large because he then sets his mind to it for a long time. I like that they have a lot of collagen in them, I can't say I've noticed that his coat is a great deal shinier but it can't hurt! I also like that these are rawhide free. I would say these are a good value as they keep him busy for a good amount of time.
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Reviewed in the United States on January 29, 2026
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