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Description
Mouse Hepatocarcinoma Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm<(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)<(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion<(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4  & Nbsp& Nbsp& Nbsp& Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A. |
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| Description |
Composition:
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| Storage Temp. | 4° C. 3months; -20° C. 1 year, see label for details |
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4.1 ★★★★★
Based on 23 reviews
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Product Reviews
★★★★★ 5
great hooks
Color: Black3pro
Strong hooks, work great, look nice. hold lots of hats!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 9, 2025
★★★★★ 5
Good product
Size: 2.5", Style: Closed Hooks
Strong magnets that stay on wall once applied. Definitely holds the weight that it says it holds on the product. Good size to hold my halters in my barn. Would buy again and I think the price was a little high for this product, but it was worth the purchase.
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Reviewed in the United States on May 26, 2026
★★★★★ 5
Versatile and Sturdy Storage Solution
Size: 2.5" & 4" - 4 Pack, Style: Open Hooks, Size: 2.5" & 4" - 4 Pack, Style: Open Hooks
The GATOR MAGNETICS Open Storage Hook has been a great (pricey) solution for adding hooks that are strong and useful. Its simple yet robust design makes it incredibly versatile for various uses around the house.
The hook feels sturdy and durable, capable of holding a surprising amount of weight without bending or warping. Installation is as easy as blinking your eyes; the really strong magnets allow for easy attachment to any magnetic surface, and the hook remains securely in place.
I've used it for hanging lights and bags in my office and bedroom, providing a neat and accessible storage solution in any setting.
The open design ensures quick access to items without the hassle of removing them from a closed loop, which is a fantastic convenience. The magnet seems to be protected well enough with a rubber surface so that I don't have to worry about damaging my tables that I have attached these hooks.
In terms of value for money, this storage hook is pricey but no competition that I've seen with the same level of durability and convenience. I'm impressed by its performance and versatility, making it a must-have for anyone looking to organize their space efficiently.
Pros:
Sturdy and durable build quality
Easy magnetic installation on metal surfaces
Versatile usage for multiple items
Convenient open design for quick access
Doesn't damage surfaces
Cons:
None that I've encountered so far! (Maybe price?)
Overall, I highly recommend the GATOR MAGNETICS Open Storage Hook for its durability, versatility, and exceptional functionality. It's a small investment that brings significant organization and efficiency to any space.
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Reviewed in the United States on November 21, 2023
★★★★★ 5
Amazingly strong and versatile
Size: 2.5" & 4" - 4 Pack, Style: Open Hooks
I saw these advertised on Instagram and immediately purchased a 4 pack. I really wanted them to work but after trying them on 4-5 different steel surfaces I couldn't'[t get any of them to stick well or hold anything heavier than a wet towel. Maybe a bad batch? A shame.
The company took the time to reach out to me after seeing my first review where the hooks wound stick to metal poles in my studio. I definitely was not expecting them to reach out to address my issues with the hooks I purchased and returned. After trying the new replacement hooks out I now could not be more impressed! They are strong, well made and easy to use. The hooks and the magnetic baskets are phenomenal and I highly recommend them. I did a test with the hooks, which held a 75 lb weight with no issues on the side of a craftsman tool box! It seems that any early product issues are fixed. The customer service is fantastic and they really took the time to address the issues and keep their customers coming back happy. Well done! Gator Magnetics is definitely a class act in my book!
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Reviewed in the United States on April 15, 2024
★★★★★ 5
Easy to use!
These work great for a metal workshop! Holds up my 6ft ladder and pressure washer hose with ease. Definitely recommend
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 20, 2026
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