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Description
Human SLC30A8 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against zinc transporter 8 (SLC30A8). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of zinc transporter 8 (SLC30A8) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Zinc transporter 8 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Solute carrier family 30, member 8 (SLC30A8), also known as zinc transporter 8 (ZNT8), is a protein encoded by the SLC30A8 gene. It is a zinc transporter involved in insulin secretion. Certain alleles of this gene may increase the risk of type 2 diabetes, but loss-of-function mutations appear to significantly reduce the risk of diabetes. The protein encoded by this gene is a zinc efflux transporter involved in the accumulation of zinc in intracellular vesicles. This gene is expressed at high levels only in the pancreas, particularly in the islets of Langerhans. The encoded protein colocalizes with insulin in secretory pathway granules of insulin-secreting INS-1 cells. Allelic variants in this gene confer susceptibility to diabetes, non-insulin-dependent diabetic mellitus (NIDDM). Several transcript variants encoding different isoforms of this gene have been identified. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.4 ★★★★★
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★★★★★ 5
This book helped me get focused on how to implement ai into my business and make money.
Format: Paperback
This book helped me get focused on how to implement ai into my business and make money.
I bought this book in early spring to get clarity on how to use AI into my day-to-day business. I read through the book quickly because it’s very easy to read. I love the back part of the book because it details step by step how to implement the techniques he uses. I thought wow now I have somebody (who’s already succeeded) to help me figure out how to implement AI. With all the information out there it’s been hard to know what to use and what not to use and what's practical and what's not practical.
From the very first page: Dan does not waste your time. I’ve read countless books over the course of my life, and this easy-to-read book stands out because it inspires and motivates the reader to take action immediately.
The author takes complex concepts, the kind that usually make your eyes glaze over—and breaks them down into clear, step-by-step processes. Instead of just telling you what to do, the author shows you exactly how to do it.
This is a practical book demands action. The author makes it clear: knowledge without action and implementation is worthless. He encourages readers to begin stating,” Don’t wait to start, just start, (do the outreach work) build the processes and practice, practice, practice, that's what this book is all about. Act first and then tweak the process, because nothing will happen if you don’t get started.
Whether you're just starting out or your business has been operational for years, this book will help you. It's not just another good book—it's a resource that helps you move your business in the right (AI Implementation) direction.
I don't often take the time to make Amazon Book recommendations. But this one? It's earned a permanent spot in my backpack, and my desk.
How can this book help you? Real, practical knowledge you can use starting today.
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Reviewed in the United States on September 12, 2025
★★★★★ 5
One word - Awesome, there is nothing like it out there
Format: Kindle
This book is one of the most valuable resources I’ve come across. From start to finish, it’s packed with actionable insights and practical advice you can immediately use. There’s no filler or fluff—every page is focused on delivering value. The author clearly knows the subject matter inside and out, and it shows in the way complex ideas are explained in such an accessible manner. Finding a book that gets straight to the point without sacrificing depth is rare, but this one does exactly that.
What impressed me was the level of detail in every section. The step-by-step explanations, including video explanations, ensure there’s no room for confusion or guesswork. Even topics that might feel overwhelming at first are broken down into manageable pieces, making it easy to follow along and build your understanding as you go. The author left no stone unturned in ensuring the reader has all the tools and knowledge they need to succeed.
I highly recommend reading this book and taking the time to apply what you learn. Its value is unmatched, and the results will speak for themselves. Whether new to the subject or looking to deepen your expertise, this book is a game-changer. It’s not just a good read—it’s a practical guide that delivers real results if you’re willing to put in the effort.
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Reviewed in the United States on January 15, 2025
★★★★★ 5
Quick and easy strategies for growth using AI
Format: Paperback
This book is full of practical tips and easy-to-understand advice, so you don’t need to be an expert in AI to benefit from it. I highly recommend it for the following reasons:
Simple, Actionable Tips: The author breaks things down into easy steps, so you can start using AI right away, even if you're a beginner.
Quick Results: This book isn’t just about learning what AI is—it’s about actually using it to grow your business fast. The tips are all about taking action and seeing results quickly.
Real-Life Examples: There are lots of examples throughout the book showing how others are using AI, which makes it easier to picture how it could work for you.
Easy to Follow: Whether you’re new to AI or have some experience, the book explains everything in a way that’s simple and clear. No jargon, just straightforward advice.
Tools for Growth: From reviving old customers back to finding new leads or making your day-to-day easier, the book gives you the tools to move your business forward fast.
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Reviewed in the United States on April 1, 2025
★★★★★ 5
A Game-Changer for Entrepreneurs – Truly Instant Impact!
Format: Paperback
As someone who's always been fascinated by the potential of AI but felt intimidated by the technical jargon, "The Instant AI Agency" by Dan Wardrope has been an absolute revelation. This isn't just another business book; it's a meticulously crafted blueprint that demystifies the "Digital Gold Rush" and shows you exactly how to stake your claim, even if you're far from a "tech nerd."
Wardrope's approach is refreshingly practical and direct. He doesn't waste time on theory; instead, he dives straight into the actionable strategies for leveraging AI to generate substantial revenue for businesses. The concepts he lays out are not only brilliant but, after reading this book, feels entirely achievable.
What truly sets this book apart is its focus on simplicity and immediate results. The "AI agent" model he outlines is a genius way to deliver immense value to clients without needing to build complex systems from scratch. I particularly appreciated how he breaks down the process of identifying high-value niches and approaching potential clients with an irresistible offer.
If you're an entrepreneur, a consultant, or anyone looking to tap into the immense power of AI to create a highly profitable business with minimal overhead and technical expertise, you must read this book. It's concise, compelling, and packed with insights that will shift your perspective on what's possible in the new digital economy. Highly, highly recommended!
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Reviewed in the United States on August 6, 2025
★★★★★ 5
The Last Book You Will Ever Need About Starting Your Own AI Business
Format: Paperback
I first purchased this book on Audible to listen to during a long road trip, thinking it would be an interesting way to pass the time. What I didn’t expect was just how engaging and eye-opening it would be. By the time I finished listening, I was so excited about what I had just learned that I immediately called my wife and asked her to order me a hard copy. I knew this was a book I needed to have on my shelf, not just to reference but to truly study.
At the time, I had been toying with the idea of starting an AI design company and creating print-on-demand products. However, this book introduced me to the concept of AI androids and their incredible potential, completely shifting my perspective. The insights and possibilities it presented made me rethink my approach and explore entirely new directions.
Beyond just being informative, the book is exceptionally well-written, with clear, concise instructions that make even complex concepts easy to grasp. Whether you're a complete beginner or someone with experience in AI, the guidance provided is structured in a way that makes it accessible to anyone willing to learn.
Although I haven’t officially started my journey yet, this book has given me the confidence and motivation to take that first step soon. I’m grateful to Dan for sharing his knowledge in such a practical and inspiring way. If you're even remotely interested in AI, technology, or the future of innovation, I highly recommend giving this book a read—you won’t regret it!
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Reviewed in the United States on April 4, 2025