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Description
Human STUB1 (E3 Ubiquitin-protein Ligase Chip) ELISA KitThe Human (STUB1) E3 Ubiquitin protein Ligase Chip ELISA Kit measures E3 ubiquitin protein ligase CHIP in samples. The plate has been pre coated with Human STUB1 antibody. STUB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human STUB1 Antibody is added and binds to STUB1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated STUB1 antibody. After incubation unbound Streptavidin
The Human (STUB1) E3 Ubiquitin-protein Ligase Chip ELISA Kit measures E3 ubiquitin-protein ligase CHIP in samples. The plate has been pre-coated with Human STUB1 antibody. STUB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human STUB1 Antibody is added and binds to STUB1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated STUB1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human STUB1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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