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Description
Human Cyclin D1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes and remove the supernatant for analysis. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, but avoid repeated freeze-thaw cycles. Pre-Assay Preparation: 1. Remove the reagent kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let stand for 15 minutes to completely dissolve, then gently mix (concentration 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 20ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube is used as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a cyclin D1 capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of cyclin D1 in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Cyclin D1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Cyclin D1 is a protein encoded by the CCND1 gene. The CCND1 gene encodes the cyclin D1 protein. This gene is located on the long arm of chromosome 11 (band 11q13). It is 13,388 base pairs long and translates to 295 amino acids. Cyclin D1 is expressed in all adult tissues, except cells derived from bone marrow stem cells (both lymphoid and myeloid lineages). Its overexpression has been shown to be associated with early cancer onset and tumor progression, and it can contribute to tumorigenesis by increasing anchorage-dependent growth and angiogenesis through VEGF production. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenate, cell culture supernatant |
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4.3 ★★★★★
Based on 10 reviews
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Product Reviews
★★★★★ 5
Great.
Size: 1 Pack, Style: USB Receiver, Color: Swift Grey
Great deal. Works great.
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Reviewed in the United States on May 24, 2026
★★★★★ 5
Great mouse
Size: 1 Pack, Style: USB Receiver, Color: Swift Grey
It synced right up and is working great
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Reviewed in the United States on June 7, 2026
★★★★★ 5
Basic mouse that works - - for now.
Size: 1 Pack, Style: USB Receiver, Color: Blue
I ordered this mouse (in blue) to replace the recently deceased exact same model that I've used for the past 2 or 3 years. I like the simplicity of the M185 mouse and mostly the best bang for your buck! I caught this one on sale for less than a ten-spot, so I thought, "why not."
I'm hoping that this one will last a bit longer than my two previous M185 models, that averaged about 3 years each. This model seems plagued by a short life span and is predisposed to mysteriously and suddenly dying, without any obvious symptoms or warning of any kind - - you're working and clicking along, going about your day, when suddenly the cursor on the computer screen freezes and that's it. Nothing, zero, nada. Funny thing, I'm very careful not to abuse or drop the mouse, and keep a fresh battery installed and watch for leakage and all that stuff - - they just stop working, and I assume it is caused by some sort of inherent electronic fault in the circuitry of the previous M185's. Due to my loyalty to the brand and model (and since it was on sale) I decided to give it one more shot. They say that "third time's a charm".
I like the fact that the M185 is simple and uncluttered with extra buttons that I would never use for my purpose, which is simple web-browsing and the occasional text document production. The feel is solid, not heavy, but not cheap feeling, either. I'm used to the shape of this mouse, which to me is quite comfortable for long sessions, another reason why I kind of wanted to stay with this model. The buttons have an audible "click", which doesn't bother me at all, and the wheel moves smoothly and also has a clicking operation (which I rarely ever use). The overall surface of the mouse has a matte finish, but like my previous models I'm sure it will eventually wear shiny spots where my fingers reside and will attain a more "well-used" look in time. Connecting it was a breeze, too. I just plugged the dongle into a spare rear usb port of my desktop, and the mouse connected instantly, no problem. The dongle can be stored safely inside the battery compartment, so you can take the whole thing out of the house if needed for use elsewhere. The speed of 1000 dpi seems just right for my purposes. The one I ordered even came with its own AA Duracell battery, which I wasn't really expecting. This mouse also enters "sleep mode" when you don't use it for a while, and I can't say how many times I've got up and left it on all night. And that's not hard to do, as I'll explain next.
The only real negative to this mouse is the absence of any easily visible status indicator of it's on / off switch. The rear housing of this mouse is matte black, including the tiny switch where you turn it on and off with your fingernail, with only a very faint "on" lettering (also black), and very hard to see with my aging eyes. The laser is not lighted or visible when on, also adding to the confusion, especially in a darkened room. So, as a workaround, I took a tiny drop of green paint and placed it down inside the switch slider to visibly help indicate the "on" position, and likewise a dot of bright orange for the "off" position. Many of Logitech's more expensive models (and a few other manufacturers) include this color-coded switch indication. I guess for the $$, they had to cut corners somewhere - - a minor complaint.
In summary, this is a good basic mouse that works - - let's hope that this one will last longer than its two predecessors.
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Reviewed in the United States on February 17, 2023
★★★★★ 3
Comfortable, but not well laid out and a bit too expensive.
Style: Right-Handed, Pattern Name: Combo, Color: Graphite
I have used a particular brand of keyboards for decades because of their features, comfort, reliability, and functionality. However, I type non-stop at my job and my employer usually just gives me run-of-the-mill equipment to use, so I always buy my own. My typing style will literally eat the keys off after about 2-3 years and 80% of the letters are gone off the keys in less than a year. I tried milking my last setup for an extra amount of time, but it was pretty bad. So I did some shopping for an ergo setup like this. I have a lot of nerve damage from my career choice so these features are important to me and after some shopping I landed on this selection. I will say I do like the feel of the keyboard and even though the mouse takes some adjustment I find it easy to use and very comfortable. So far the keys are holding up well to my abuse. I like the comfort of the wrist rest as well. The only real negatives are the following:
I don't care much for the keyboard layout, the arrow keys are in a goofy spot, and the home end pg up and down keys are in a terrible place as well. It took forever for me to get used to the backspace and delete key locations too. I'm adjusting okay but it has not been super easy. Also, I don't like the position of the function keys to activate the other operations mapped to the F keys. Their selection for what these functions are is not for someone in business. It seems that it is for a teenager. An emoji key? Seriously?
The media keys are pointless as there is no forward or back skip feature and it takes both hands to use them . You have to take your eyes of what you are doing and look at the keyboard just to use these features. The function and windows key should be switched in position as well. Being a PC and not a Mac person may be one of my issues because I don't like the labeling of "start/opt" A feature of this particular unit is sometimes helpful and sometimes not. It seems to have an overactive "enter" key function. Randomly, it hits enter without any input from me. Granted I have a lot of papers flying around but it can happen at inopportune moments, costing me extra keystrokes to undo what was done by this issue. Sometimes it is helpful but I prefer to be in control of what my keyboard is doing and not the other way around. I do like the action of the keys and the comfort, like I said more of my issues are related to specifically how I use it. I have not had any connection issues, battery issues, or any other technical issues. It appears to be fairly reliable and accurate. The last thing I would say is that this setup really seems overpriced. Not sure what that is about but if I had paid for it rather than my employer I would not consider it worth what I paid. With the pluses and minuses I would rate this as a decent purchase but not an ideal piece of equipment for my specific use.
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Reviewed in the United States on April 20, 2026
★★★★★ 4
Vast improvement over traditional mice
Style: Left-Handed, Pattern Name: Mouse, Color: Graphite
This review is primarily for the left handed version. When Logitech introduced the left handed Mouseman in 1991, it was the first and only ergonomic mouse for left handers. Merchants refused to dedicate shelf space to it, so it was short lived.
The latest version is leaps and bounds above the original and is the most comfortable and natural feeling mouse that I've ever used. At first, it was awkward to use. After about 20 seconds, it felt natural, and traditional mice started feeling very awkward. This mouse lets you keep your arm and wrist in a natural position, whether you are using it as a desk mouse or a couch mouse.
The primary button is the index finger button, whether you use the left or right handed version. The Logitech software doesn't have a true setting for swapping buttons, and uses the Windows setting. So if you had your buttons swapped for your old mouse, you need to unswap them. It also means that your old mouse becomes a right handed mouse on that computer. There's no way of having both set to left handed.
There's a switch to change the sensitivity. The normal one lets you do work needing fine movements, such as drawing programs. The other setting makes it more sensitive to movement, which means more mouse movement with less hand movement. It's also a good feature if you have limited desk space or are using an airplane tray. This lets you avoid having to use the setup screens to change sensitivity.
The scroll wheel is smooth and accurate. It also works for horizontal scrolling if used in conjunction with one of the buttons on the thumb side. It takes getting used to, and is still awkward for me. Those buttons are forward and back buttons by default, so don't use it while editing something on a web page until you are used to it, or you could end up losing everything. A problem is that their Logi software doesn't let you set the scroll increment. It can be done with the Windows mouse settings, but you shouldn't have to go two places to set what the mouse does. Pressing the middle button changes from moving the pointer up and down to scrolling the page up and down. Unfortunately, there's no speed adjustment. The round symbol that pops up should really be a longer vertical symbol that allows greater control over speed.
The mouse includes a Logibolt receiver that can plug into a USB port, or you can use the mouse directly with your computer's Bluetooth if it meets the specs.
If you use it with your computer's Bluetooth, you remove the tab from the mouse so the battery connects, and then use your computer's Bluetooth configuration. That will save you a USB port. If you need to use the mouse to set up Bluetooth, use the receiver, and then store it inside the mouse once you have your computer's Bluetooth set up.
If you use the receiver, plug it into a USB port first, then pull the tab. If you want to be able to use both, there's a button on the bottom to let you set it for up to three different devices. So if it's set for your computer's Bluetooth, and want to set up the receiver to use on another computer, plug in the receiver, touch the button on the bottom of the mouse (the light will move from 1 to 2) and you will be paired. You can also use the third setting for another device. If you paired it to both using the same computer, you can unplug the receiver and use the button to go back to setting 1.
If you have a desktop computer, you will want to have the receiver paired, and can use it if you need to change a bios setting, since Windows won't be loaded. So it's good to pair it, even if you will just store it inside the mouse. It would be good to pair it anyway with any computer, so it's ready to go if you ever need it.
There's no instruction manual included, nor do they give you a link to one in the configuration software. The QR code takes you to the software download page, but doesn't give a link to the online manual. So some of this wasn't clear to me without searching online. Their original Mouseman came with a 148 page manual and a 48 page getting started guide. The new one comes with pictorial printed on the cardboard it's wrapped in. Something in between might have been nice, even if it were just a link to the manual.
Update: 18 months later, it's still working well but the battery is down to 5%. I'm changing the battery.
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Reviewed in the United States on November 14, 2022