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Description
S-RMab® BOB-1 Recombinant Rabbit mAb (SDT-R115)Product Specification Host Rabbit Antigen BOB 1 Synonyms OBF 1, OCA B, OCT binding factor 1 Immunogen N A Location Nucleus Accession Q16633 Clone Number SDT R115 Antibody Type Rabbit mAb Application WB, IHC P, ICC, ICFCM, IP Reactivity Hu Purification Protein A Concentration 0. 25 mg ml Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05% BSA, 0. 03% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution, 20 C as
Product Specification
| Host | Rabbit |
| Antigen | BOB-1 |
| Synonyms | OBF-1, OCA-B, OCT-binding factor 1 |
| Immunogen | N/A |
| Location | Nucleus |
| Accession | Q16633 |
| Clone Number | SDT-R115 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P, ICC, ICFCM, IP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | null |
| WB | 1:500 | null |
| ICC | 1:250 | null |
| IP | 1:25 | null |
| ICFCM | 1:250 | null |
Background
BOB-1 interacts with the sequence-specific DNA-binding POU transcription factors (named after the founding family members PIT1, OCT1/2, and UNC86), the ubiquitously expressed OCT1 (POU2F1) and lymphoid-specific OCT2 (POU2F2) [PMID: 33864944IF: 9.2 Q1 ]. As a transcriptional co-activator, BOB-1 itself does not bind DNA but is rather recruited into transcriptional regulation via interaction with DNA-bound POU-domain transcription factors OCT1 and OCT2. The POU-domain is a unique bipartite structure allowing DNA recognition with remarkable flexibility [PMID: 12213595, PMID: 29335749].
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Exchange/Return Notes
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