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Description
Alexa Fluor® 488 Rat Anti-Mouse IgD Antibody (S-R622)Product Specification Host Rat Antigen IgD Synonyms Ig delta chain C region membrane bound form Location Cell membrane Accession P01882 Clone Number S R622 Antibody Type Rat mAb Isotype IgG2a,k Application FCM Reactivity Ms Positive Sample C57BL 6 mouse splenocytes Purification Protein G Concentration 0. 2 mg ml Conjugation Alexa Fluor 488 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300 Stability & Storage 12 months from date
Product Specification
| Host | Rat |
| Antigen | IgD |
| Synonyms | Ig delta chain C region membrane-bound form |
| Location | Cell membrane |
| Accession | P01882 |
| Clone Number | S-R622 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
IgD is a monomeric antibody isotype that, in humans, is primarily co-expressed with IgM on the surface of naïve B cells where it functions as an antigen receptor whose cytoplasmic tail participates in B-cell receptor signaling to modulate activation, survival, and differentiation; although it constitutes only about 0.25 % of serum immunoglobulin, secreted IgD appears to bind basophils and mast cells via galectin-9 to promote antimicrobial and inflammatory responses at mucosal surfaces, while its heavy chain constant region contains three immunoglobulin-like domains and undergoes extensive alternative splicing and class-switch recombination that together provide evolutionary flexibility and fine-tuned regulation of immune responses.
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