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Description
Mouse EVL ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an Enah/Vasp Like Protein (EVL) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Enah/Vasp Like Protein (EVL) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Enah/Vasp Like Protein ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Enah/Vasp-like protein (EVL), also known as Ena/vasodilator-stimulated phosphoprotein-like, is encoded by the EVL gene and is a member of the Ena/VASP protein family. Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeletal remodeling and cell polarity, such as axon guidance and lamellae and filament dynamics in migrating cells. EVL enhances actin nucleation and polymerization. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.3 ★★★★★
Based on 23 reviews
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Product Reviews
★★★★★ 4
Pretty good product
Style: Single Arm, Size: 13"-34"
Assembly of the actual stand is easy, mounting to a monitor is not because of industrial design fluff. Gotta make the side you DON'T see look "sleek" and "smooth" and "organic" and "integrated" because...reasons. While this is on the manufacturer(s) of the monitor itself, what semi-modern monitor uses 100x100 VESA anyway?
The included long screws are too long. Even using the plastic spacers they'll bottom out with a 2mm gap; add washers and it's a lever arm and not solid or confidence-inspiring. I used a die grinder to remove the excess material closer to 75x75 and used the included short screws, perfect. So either include 25mm screws instead of the 30mm (but then the spacers would be too long), or just update the mount plate to 75x75 only, and throw 100x100 "compatibility" into the dustbin of history where absolutely no one will miss it.
Arm does move/adjust smoothly and is holding a 34" widescreen where I left it 2 days ago, so it IS a great product once it's properly fitted.
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Reviewed in the United States on January 22, 2026
★★★★★ 5
Great monitor arm stand.
Style: Single Arm, Size: 13"-34"
Very happy with this product. It's sturdy and easy to install. Love the tilt and swivel feature. The fittings for the stand are very well made. I suggest this for the quality and the price.
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Reviewed in the United States on May 3, 2026
★★★★★ 5
Easy to set up & works great
Style: Single Arm, Size: 13"-34"
It was super easy to get set up, came with all the hardware ypu need to install multiple ways. Holds my monitor securely and is still easy to move around. The adjustments are easy to access and have plenty of variation. Great purchase, such a reasonable price.
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Reviewed in the United States on May 16, 2026
★★★★★ 5
I love it!!
Style: Dual Arms, Size: 17"-33"
I love this product. I do a lot of gaming and you tube watching. At the same time a lot. With this I keep my desktop clear so I can have my notebooks there and can still have both my monitors up and running. Another plus is if I decide to sit on the couch and play the console I can turn the screen a full 180 degrees so I can setup you tube and play games on the T.V. using the console.
The product itself feels to be of decent quality. It is not all plastic. The clamp is nice and strong.
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Reviewed in the United States on May 24, 2026
★★★★★ 3
Gets the job done. Just barely.
Style: Dual Arms, Size: 17"-33"
It holds up my monitor, but just barely, and without any confidence or finesse.
I had to move on from my previous arm mounts, the HUANUO. Those claimed to hold up to a 30 inch monitor and support 20 pounds. When I upgraded to a Samsung Odyssey Neo G8, it could no longer perform its duties. The gas shock was up to task, but the final adjustment piece that tilts it up or down was a hard down.
Queue this piece of hardware! 22 pounds is more than 20, and 32 is more than 30. This time I'm not even technically out of spec. The clamping force seems more than adequate to hold this to my desk. I will, at this point, basically walk you through how I feel about this mount, based on the order of installation instructions.
The way it goes together means that you will have to pull your desk out to maneuver the clamp in place before snugging it back up to the wall, a point in the HUANO's favor since it had a clever leveraging mechanism that lets you hang it with your desk still against the wall.
The way cords route through the arms close to the base means that you have to route them before you put the swivel arm on the base. This is because it has a screw at the front and back to secure the cover, and the angle/height are such that you cannot fit a screwdriver into the bottom one after you mount it. While not impossible to do with it installed, it would involve a right-angle screwdriver. They provide one of those, but the screwdriver bit of it is on the long shaft, and you would need it on the short shaft to get to that screw.
The way the swivel arms mount on the base is less than ideal as well. The arm slips onto a robust metal hub, and you tighten a screw that pushes against a plastic tab that makes it harder or easier to move. The location for this screw faces the wall. So you must turn the arm at an extreme angle to make it tighter or looser. Not a big deal, but noticeable.
Top arms go on smoothly, cable routing is simple and easy. No complaints about this section.
Mounting the monitor. The instructions are to mount the plate to the monitor, and then slide the plate onto the holder and secure it with the screw. I did this for neither of my monitors, although I tried it for my second. My big monitor went smoothly by mounting the bracket ahead of time and just forcing the arm down into position while my monitor rested on my desk. I tried this for my second bracket, but it was much harder to do with the monitor in a vertical orientation. So then I tried to do it the way the instructions wanted. I would have been able to do so, but my monitor has the mounting location set into the back of the monitor. This means that you can't mount it flush and then slide it on because you don't have clearance. So I tried to use the spacers. The spacers aren't spacious enough and the screws bottomed before snugging. While I'm not saying that solution wouldn't have technically worked, I instead reverted back to mounting the bracket first. This time I laid my monitor flat on the desk, reduced the tension in the shock greatly, and placed it into position that way.
At the end when it's complete and setup? It works. It will hold the G8. But just barely. The G8 uses an adapter to go from the monitor mounting points to VESA 100. This adapter sticks out quite a bit from the monitor, which means the weight is further away from the mounting point. Getting the tilt adjustment to hold involved a bigger allen wrench and cranking it further than it seems like it was meant to go. It groaned, but it held. It wants to dip if you mess with it, so I crank it again. Will it one day totally fail on me and point my monitor at my desk? Maybe. But it works on day 1, so that's a start.
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Reviewed in the United States on February 29, 2024
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