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Description
IL-4 Protein, RatProduct Specification Species Rat Synonyms Interleukin 4; IL 4; B cell IGG differentiation factor; B cell growth factor 1; B cell stimulatory factor 1 (BSF 1); Lymphocyte stimulatory factor 1; Accession P20096 Amino Acid Sequence His23 Ser147 Expression System HEK293 Molecular Weight 17 20 kDa (Reducing) Purity >95% by SDS PAGE; >90% by HPLC Endotoxin <0. 1EU g Conjugation Unconjugated Tag No Tag Physical Appearance Lyophilized Powder Storage Buffer
Product Specification
| Species | Rat |
| Synonyms | Interleukin-4; IL-4; B-cell IGG differentiation factor; B-cell growth factor 1; B-cell stimulatory factor 1 (BSF-1); Lymphocyte stimulatory factor 1; |
| Accession | P20096 |
| Amino Acid Sequence | His23-Ser147 |
| Expression System | HEK293 |
| Molecular Weight | 17-20 kDa (Reducing) |
| Purity | >95% by SDS-PAGE; >90% by HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | Cytokine. 2015 Sep;75(1):3-7. |
Background
Interleukin-4 (IL-4) is a 13–18 kDa, glycosylated, four-α-helix cytokine that was originally described in the rat as “B-cell stimulatory factor-1”. It is synthesized with a 24-aa signal peptide and, once cleaved, the mature rat protein shares ≈41 %, 43 % and 59 % amino-acid identity with bovine, human and mouse IL-4, respectively; cross-species bio-activity is negligible, making rat-specific reagents essential for in-vivo and ex-vivo work. In the rat, IL-4 is produced chiefly by Th2-polarized CD4⁺ T cells, mast cells, basophils and eosinophils, and signals through two receptor heterodimers: the type-I receptor (IL-4Rα + common γ-chain) found on haematopoietic cells, and the type-II receptor (IL-4Rα + IL-13Rα1) expressed on non-haematopoietic cells and shared with IL-13. Engagement of either complex triggers JAK1/3-STAT6 phosphorylation and, secondarily, the IRS-2/PI3K pathway, leading to characteristic Th2 effector functions: IgE and IgG1 class-switching in B cells, alternative (M2) activation of macrophages, mucus production by airway epithelium, and chemotaxis of eosinophils and mast cells. Consequently, IL-4 is the central driver of allergic airway inflammation in rat models of asthma and is routinely used to skew splenocyte cultures toward a Th2 phenotype in vitro.
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