SKU: 74715849945

Human IL-1α ELISA Kit

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Description

Human IL-1α ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti human il 1 Capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The il 1&alpha in the sample;

Product Specification

Description

Detection Principle:
This kit uses double antibody sandwich ELISA technology. Specific anti-human il-1α Capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The il-1&alpha in the sample; Bound to solid-phase antibody and detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color reaction and il-1&alpha in the sample; There is a positive correlation between the concentration of. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: Pre coated 96 well plates, standards, il-1α One copy of detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.52pg/ml

Detection Range: 7.81-500 pg/ml

Recovery Range: 81-106%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 1 (IL-1)It is a 17kDa extracellular polypeptide, which is divided into il-1α And il-1β Both proteins. Il-1α Is a pleiotropic cytokine encoded by the IL1A gene in humans. Il-1α It is mainly produced by activated macrophages, neutrophils, epithelial cells and endothelial cells. It has metabolic, physiological and hematopoietic activities and plays a central role in regulating the immune response.

IL-1α It can interact with HAX1 and NDN, but the synergy with TNF is the most clinically relevant. Il-1α And TNF are both acute phase cytokines with Pro febrile and inflammatory effects.

At present, relevant scholars are evaluating il-1α Whether it can be used as a potential tumor treatment marker in clinical trials. Blocking il-1α Activity has potential therapeutic effects on skin diseases, such as acne.
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SKU: 74715849945

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Ryan
Lexington, US
★★★★★ 1
One Star
Format: Paperback
Exactly the same as the free PDF I downloaded online for my student, only this costs $26 plus tax.
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Reviewed in the United States on April 3, 2017
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Andres Hoyos
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★★★★★ 5
Excellent customer service
Format: Paperback
Totally recommendable.
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Reviewed in the United States on August 26, 2019
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Amazon Customer
Battle Creek, US
★★★★★ 5
Five Stars
Format: Paperback
This helped a lot for us to prepare for the SLATE exam.
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Reviewed in the United States on August 23, 2016
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Amazon Customer
Belleville, US
★★★★★ 5
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This is great! I like this one.
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Reviewed in the United States on June 22, 2016
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Ng Meowster
Lexington, US
★★★★★ 2
Good to have if you've never seen the iTEP exam, but don't rely on it to boost your scores
Format: Paperback
This book is good in that it comes with two practice tests: one in the beginning of the book, one in the end of the book. The grading rubric is helpful too. However, the actual guide part of the book, doesn't help too much with how to improve test scores. What I like least about this guide is that it won't tell you which questions you answered incorrectly on the practice exams. A good test prep guide will tell you what you answered incorrectly, and will tell you what logic to follow to get to the correct answer. In the reading passages, it would be nice to know where I can find evidence to justify the "correct" answer. In the grammar section, it would be nice to know which rule I'm violating. Any test guide should always come with some testing strategies. I.E. If this question is taking up too much time, skip it and go to the next one. This guide lacks simple strategies like that. Simple strategies like that could be useful when incorporated into this guide because there are certain sections on the exam that you can go back to previous questions, but some sections where the "back" button is disabled. Overall, if you've never seen an iTEP exam before, this book is certainly helpful in that it will give you an idea of what you will be testing for. However, this book alone won't help you achieve a score of 6. The table of contents itself will tell you there are only 86 pages of actual content covering test material. That's not enough to cover how to excel in the exam.
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Reviewed in the United States on January 27, 2015

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