SKU: 9818138884

Human TNF-α High Sensitivity ELISA Kit

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Description

Human TNF-α High Sensitivity ELISA KitProduct Specification Synonym APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte derived; TNFA; TNF A; TNFalpha; TNF alpha; TNF alphacachectin; TNFATNF, macrophage derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor alpha Description Testing Principle This

Product Specification

Synonym APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
Description

Testing Principle:
This kit uses double antibody sandwich ELISA.Specific anti-human TNF-α Capture Antibody is precoated on a high affinity conjugate plate.  Standard substance, sample to be tested and biotin labeled detection antibody were added successively into the plate well of the enzyme label. After fully shaking and mixing, the samples were placed at room temperature for a 2-hour incubation process. The TNF-α in the samples was bound to the solid phase antibody and detection antibody.  After washing thoroughly to remove free and unbound ingredients, horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is added.  After washing, signal enhancer was added for incubation. After washing and removing unbound substances, SA-HRP was added again.  After washing again, TMB color substrate was added and incubated in dark at room temperature for color development.  The depth of color reaction was positively correlated with the concentration of TNF-α in the sample.  The termination solution was added to terminate the reaction, and the absorbance value was measured at 450 nm detection wavelength (correction wavelength 570-630 nm) using a microplate analyzer.  


Detection type:Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity:100 μl

Kit composition:pre-coated 96-well plate,Standards,Standard, TNF-α Detector Antibody, Standard Diluent, Detector Buffer, Signal Enhancer, Signal Enhancer Diluent, TMB Color Substrate, Washing Solution, Termination Solution, SA-HRP, Sealing Plate Mask and Manual  

Sensitivity:0.16 pg/mL

Detection range:1.56-100 pg/mL

Recovery rate:100-108%

Storage:2-8℃

The standard curve

 

Background:

Tumor necrosis factor α(TNF-α), also known as cachexin and TNFSF1A, is an adipokine that is involved in systemic inflammation and is one of the cytokines that stimulate acute phase responses. It is produced mainly by activated macrophages, but can also be secreted by other types of cells, such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It has a variety of regulatory functions in the immune response, but also can be used as a potential pyrogen. In response to stimuli (infectious agents or tissue damage), TNF-α circulates throughout the body, activates neutrophils, alters the properties of vascular endothelial cells, regulates metabolic activity in other tissues, and exhibits tumor-killing activity by inducing local coagulation. TNF-α plays an important role in the pathogenesis of inflammation in joint tissues and other tissues. Recently, more and more information indicates that TNF-α is also involved in the pathogenesis of AIDS. The determination of TNF-α is also useful for transplantation studies.  

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SKU: 9818138884

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Shannon VanDuyne-Johnson
Dallas, US
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Rodeo
Color: Brown, Size: X-Large
Perfect fit
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Reviewed in the United States on April 30, 2026
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★★★★★ 4
Color bleed
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Nice vest but it ruined my white shirt. Color bleed and little black balls all over the place
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Reviewed in the United States on November 2, 2025
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lucy
Massapequa, US
★★★★★ 5
Great for the price.
Color: Brown, Size: X-Large
I bought this vest for my brother in the Philippines. He like a vest with a fringe or tassel, and this is the only item that has it. I am rating it a 5 star because of a quick replacement for the defective item that I received at first. The materials used are thin, however, the vest still looks great. I hope they will last a bit longer after several washing.
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Reviewed in the United States on January 1, 2024
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T.O. Joseph
San Leandro, US
★★★★★ 1
Cheap-poorly made junk
Color: Coffee, Size: XX-Large
I needed a vest with some fringe for a costume party on 11/24/23. I looked at many others but decided on this one due to the fact it would arrive on Wednesday instead of Friday, the day of the party. I beyond disappointed in the quality and fit of this vest. I knew that it was faux leather but it was the worst faux leather type material I had ever seen. The fit was terrible, said 2xl but 1xl tops. It was probably my worst purchase ever on Amazon. Save your money and do not purchase this vest.
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Reviewed in the United States on November 30, 2023
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Lowell, US
★★★★★ 3
Thin
Color: Light Khaki, Size: 3X-Large
Runs somewhat small Thin material
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Reviewed in the United States on November 4, 2024

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