SKU: 78532156706

Rat IFN-γ High Sensitivity ELISA Kit

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Description

Rat IFN-γ High Sensitivity ELISA KitProduct Specification Description Testing Principle This kit uses double antibody sandwich ELISA. Specific anti rat IFN Capture Antibody is precoated on a high affinity conjugate plate. Standard and test samples were added successively into the well of the plate, and the samples were fully shaken and mixed and placed at room temperature for 2 hour incubation. The IFN in the samples bound to the solid phase antibody. Wash thoroughly to remove free and

Product Specification

Description

Testing Principle:
This kit uses double antibody sandwich ELISA.Specific anti-rat IFN-γ Capture Antibody is precoated on a high affinity conjugate plate. Standard and test samples were added successively into the well of the plate, and the samples were fully shaken and mixed and placed at room temperature for 2-hour incubation. The IFN-γ in the samples bound to the solid phase antibody. Wash thoroughly to remove free and unbound components, then add a biotinylated detection antibody and incubate. After washing again, horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is added. After washing, signal enhancer was added for incubation. After washing and removing unbound substances, SA-HRP was added again. After washing again, TMB color substrate was added and incubated in dark at room temperature for color development. The depth of color reaction was positively correlated with the concentration of IFN-γ in the sample. The termination solution was added to terminate the reaction, and the absorbance value was measured at 450 nm detection wavelength (correction wavelength 570-630 nm) using a microplate analyzer.

 

Detection type:Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity:100 μl

Kit composition:pre-coated 96-well plate,Standards,Biotin labeled IL-17A detection antibody, Standard Diluent, Detection Buffer, Signal Enhancer, Signal Enhancer Diluent, TMB Color Substrate, Wash Solution, Stop Solution, SA-HRP, Seal Plate Mask, and Instructions.

Sensitivity::0.26 pg/mL

Detection range:3.13-200 pg/mL

Recovery rate:73-137%

Storage:2-8℃

The standard curve:

Background:

Interferon γ (IFN-γ), a soluble dimer cytokine, is the only member of type II interferon.It is secreted by natural killer cells (NK) and natural killer T cells (NKT) and plays a role in innate immunity.It is secreted by CD4Th1 and CD8 cytotoxic T cells during antigen-specific immunity.IFN-γ or type II interferon plays an important role in innate A immunity and adaptive immunity against viral and certain bacterial and protozoan infections.IFN-γ is an important activator of macrophages and an inducer of type II major histocompatibility complex (MHCII) expression.Abnormal expression of IFN-γ is associated with many autoinflammatory and autoimmune diseases.In addition to direct inhibition of viral replication, IFN-γ is important to the immune system in terms of its immune-stimulating and immune-regulatory functions.
IFN-γ is a marker of disease in many different pathological conditions, including infections, autoimmune diseases, transplant rejection, allergic reactions, and diabetes mellitus.

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SKU: 78532156706

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Rosario Salazar
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Reviewed in the United States on May 27, 2026
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Nll
Grantham, US
★★★★★ 4
Good Compact Design
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Attractive design and space to store the dish brush and or sponge. My only criticism is that the pump spout is quite narrow so the liquid soap skims over and not that much is released with each pump.
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Reviewed in the United States on January 14, 2026
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Squash Master
Bozeman, US
★★★★★ 5
Good quality.
Color: Black
This is a very good quality and durable product. Nice addition to my kitchen sink top.
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Reviewed in the United States on February 21, 2026
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anonymous
Bozeman, US
★★★★★ 5
Looks great!
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Really nice looking and handy to have.
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Monika
Chelsea, US
★★★★★ 1
Bad product, cannot recommend
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This product didn't work well at all. The material gets smudged and dirty extremely easily and it's impossible to get it clean. It's also very heavy, but not in a good way. The pump shoots out dish soap very far and makes a mess on the counter. Even when I hold the sponge or brush right by the pump, the dish soap still went all over the place. The pockets for brush and sponge did not work well either. Even the taller back pocket was way too short to hold a normal size dish brush. I ended up throwing the item in the trash.
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Reviewed in the United States on July 27, 2025

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