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Description
G-CSF Protein, MouseProduct Specification Species Mouse Synonyms Granulocyte colony stimulating factor Accession P09920 Amino Acid Sequence Val31 Ala208 Expression System E. coli Molecular Weight 19kDa Purity 95% by SDS PAGE&RP HPLC Endotoxin <0. 1EU g Conjugation Unconjugated Tag No Tag Physical Appearance Lyophilized Powder Storage Buffer 20 mM PB, 150mM NaCl, 5%pH 6. 5 Reconstitution Reconstitute at 0. 1 1 mg ml according to the size in ultrapure water after rapid
Product Specification
| Species | Mouse |
| Synonyms | Granulocyte colony-stimulating factor |
| Accession | P09920 |
| Amino Acid Sequence | Val31-Ala208 |
| Expression System | E.coli |
| Molecular Weight | 19kDa |
| Purity | ＞95% by SDS-PAGE&RP-HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20 mM PB, 150mM NaCl, 5%海藻糖，pH 6.5 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1.FEBS Open Bio. 2022 Jul;12(7):1268-1285. Epub 2022 Jun 9. |
Background
As key factors in hematopoietic development, G-CSF/GM-CSF are well-known facilitators of myelopoiesis and mobilization of hematopoietic stem cells (HSCs). In addition, these two cytokines can also promote or inhibit tumors, dependent on tumor type. In multiple cancer types, hematopoiesis is greatly enhanced and abnormal lineage differentiation is induced by these two cytokines. Numerous preclinical studies have reported a pro-tumour role for granulocyte colony-stimulating factor (G-CSF) that is predominantly mediated by neutrophils and MDSCs, the major G-CSF receptor expressing populations. Granulocyte-Macrophage colony stimulating factor (GM-CSF) and Granulocyte colony stimulating factor (G-CSF) are cytokines involved in the differentiation of bone marrow progenitor cells into myeloid cells. They also activate mature myeloid cells to mediate a variety of antimicrobial activities and inflammatory responses. Recombinant GM-CSF and G-CSF proteins have been used to treat various diseases including cancer and hematopoietic diseases and to isolate peripheral blood progenitor cells for bone marrow transplantation.
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