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Description
Mouse CPT1A ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against carnitine palmitoyltransferase 1A, Liver (CPT1A). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of carnitine palmitoyltransferase 1A, Liver (CPT1A) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Carnitine Palmitoyltransferase 1A, Liver | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Carnitine palmitoyltransferase 1A, also known as CPT1, is encoded by the CPT1A gene. Mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (located in the outer membrane and detergent-labile) and carnitine palmitoyltransferase II (located in the inner membrane and detergent-stable), along with carnitine-acylcarnitine translocase. It is a key enzyme for carnitine-dependent transport across the inner mitochondrial membrane, and its deficiency results in reduced fatty acid β-oxidation rates. Alternative splice transcript variants encoding different isoforms of this gene have been identified. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.1 ★★★★★
Based on 19 reviews
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Product Reviews
★★★★★ 5
Absolutely Worth The Money
Scent: Graphite Gray_4 Pcs
These things are great. The soap stays in the bag and it acts as a natural exfoliant. It's not harsh and it works for my sensitive skin. I have psoriasis and this thing does not agitate it at all. I've never owned a bag for my soap but, it really works. The cost was low and, now this is how I shower. Much simpler than working a loofah and all that liquid soap together. I would recommend to a friend. Great value. The length is big enough to fit any bar of soap known to man. There's no soaps gun build up and no smell to them. They just smell like the soap that contains them. They feel durable and I'm positive I couldn't rip one in halfway hulk Hogan entering the wrestling ring.
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Reviewed in the United States on January 19, 2026
★★★★★ 4
Perfect for the men in your life!
Scent: Graphite Gray_4 Pcs
Honestly this is a great product for anyone but I bought one set to give to my 17yr old nephew who loves using the Dr.Squatch soap bars. The other set I bought for my soon tonbe 12yr old son. I bought him a charcoal soap bar for exfoliating & the bag is perfect for him. Lathers up well & has just the right about of coarseness to it to help scrub dry skin away. The bar keeper we use for my soap or honestly for my razor that seems to keep falling off its holder.
The bags are quite a bit bigger then I would expect for the fact a bar of soap goes in it. It would fit easily 2-4 soap bars. Not sure why that much room is needed but none the less, great product
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Reviewed in the United States on January 29, 2021
★★★★★ 5
Bigger then I thought it would be.
Scent: Graphite Gray_4 Pcs
The only complaint I have is that in the picture it looks like its about the size of a normal bar of soap. In reality though you could fit like 4-5 bars of soap in this thing, it's way bigger then I thought it would be, in fact it's huge. At first I thought this was a negative thing, but after using it a few times I realized that with the insert they give you it's fine and the soap basically stays in place because of the insert.
Quality: Seems to be decent quality
Value for money: Yeah I would say it's a decent value
Roughness: Doesn't seem too rough
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Reviewed in the United States on July 10, 2025
★★★★★ 5
A Winter Essential I Keep Reaching For
Scent: Graphite Gray_4 Pcs
I love these body scrubbers, especially now during the winter season when exfoliating dry skin is a must. The texture is just right and scrubby enough to smooth out rough spots, yet gentle on sensitive areas. I also appreciate how the pouch holds leftover soap bits so nothing goes to waste. The graphite gray color looks sleek in the shower, and the lift pads help the scrubbers dry quickly and stay fresh. They lather beautifully and make my skin feel noticeably softer after each use. This set has quickly become one of my favorite simple self-care upgrades.
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Reviewed in the United States on December 2, 2025
★★★★★ 5
Durability and cleanliness
Scent: Graphite Gray_4 Pcs
I use the soap saver pouch everyday. I honestly don’t use it for what it’s intended for besides cleaning my body. I use body wash instead of soap bars so it defeats the purpose. But this review isn’t about that. It’s more about the durability and feeling of cleanliness after using it. I don’t need to apply a lot of pressure because it is abrasive. So a light rub with body wash does the job. And if you’re to use it on your face like I do, you have to use even LESS pressure because face is MUCH more sensitive. I even wash my hair with it (I’m almost bald) but I have dandruff. I don’t have to use the shampoo anymore after scrubbing my head with this. Anyways I feel the soap savor pouch works very efficiently in getting all the dead skin off my body. I love using this product over a washcloth or those other products. WARNING: Not good to use for sensitive skin. Product is abrasive and will irritate sensitive skin.
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Reviewed in the United States on June 10, 2021
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