Antibody Glycan Site-specific Conjugation Kit

The antibody site-specific conjugation kit developed based on antibody glycan editing technology utilizes the N-297 conserved glycan in the CH2 region of the IgG heavy chain of the antibody to mediate site-specific conjugation. The site-specific modification is achieved using glycosidase and glycosyltransferase.

This kit enables rapid site-specific conjugation of monoclonal antibodies, overcoming the drawbacks of conventional conjugation methods such as random conjugation sites, heterogeneous products, or the need for antibody engineering. This kit provides a complete set of reaction reagents for antibody azidation modification and can be completed within 2 days.

As shown in the figure below, first, the sugarase EndoS is used to expose the acetylgalactosamine (blue square) on the conserved N-glycan of the constant region of the monoclonal antibody. Then, the mutant of bovine galactosyltransferase (b.GalTY298L) is used to link the acetylated galactosamine with azide functional groups (yellow square - N3) to the acetylgalactosamine. Subsequently, the copper-free catalyzed acetylenecyanide cycloaddition reaction (such as SPAAC) can be used to link biotin, fluorescein or toxin molecules, etc. to the monoclonal antibody.

Product features:
No need to modify the antibody, without damaging the antibody structure.
The product is uniform, with high and stable coupling efficiency.
Antibodies with glycosylation modification can all be applied, and they are compatible with various linkers and drugs.