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Description
TOP2A Recombinant Rabbit mAb (S-513-107)Product Specification Host Rabbit Antigen TOP2A Synonyms DNA topoisomerase 2 alpha; TOP2 Immunogen Synthetic Peptide Location Cytoplasm, Nucleus Accession P11388 Clone Number S 513 107 Antibody Type Recombinant mAb Isotype IgG Application WB, IHC P, ICC Reactivity Hu, Ms, Rt Positive Sample SK BR 3, A549, A431, HeLa, Neuro 2a, mouse testis Purification Protein A Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage
Product Specification
| Host | Rabbit |
| Antigen | TOP2A |
| Synonyms | DNA topoisomerase 2-alpha; TOP2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P11388 |
| Clone Number | S-513-107 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | SK-BR-3, A549, A431, HeLa, Neuro-2a, mouse testis |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:2000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
TOP2A (DNA topoisomerase IIα) is a crucial nuclear enzyme highly expressed during the S and G2/M phases of the cell cycle, responsible for resolving topological challenges arising from DNA replication and chromosome segregation; it functions by creating transient double-strand breaks in DNA, allowing another DNA duplex to pass through the gap before resealing the ends, thereby effectively relieving supercoiling tension, decatenating interlinked DNA rings, and ensuring the accurate separation of sister chromatids during anaphase. As a hallmark of cell proliferation, TOP2A exhibits high activity in rapidly dividing tissues, and its dysfunction can lead to genomic instability and chromosomal aberrations; furthermore, it serves as a primary target for important anticancer drugs such as anthracyclines (e.g., doxorubicin) and epipodophyllotoxins (e.g., etoposide), which exert their cytotoxic effects by stabilizing the TOP2A-DNA covalent complex to induce lethal double-strand breaks, ultimately inhibiting tumor cell growth.
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